Expression of acquired macrolide resistance genes in Haemophilus influenzae
Expression of acquired macrolide resistance genes in Haemophilus influenzae
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DOI：
10.1093/jac/dkx290
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发表时间：
2017-12-01
影响因子：
5.2
通讯作者：
Tristram, Stephen G.
中科院分区：
文献类型：
--
作者：
Atkinson, Christopher T.;Kunde, Dale A.;Tristram, Stephen G.
目的：目的：研究获得性大环内酯类耐药基因（AMRGs）在非分型流感嗜血杆菌（NTHi）中表达的表型效应。流感病毒Rd。通过肉汤微量稀释法测定合适转化体的红霉素和阿奇霉素MIC。结果：转化子中所有AMRG基因均得到表达。AMRGs之间的一些表达差异是明显的，但所有基因的表达与红霉素和阿奇霉素MIC与未转化的H.结论：虽然在NTHi的WT人群中建立H1N1基因仍然存在争议，但H.流感病毒能够表达erm。表达可能与随后的大环内酯类药物的敏感性降低，在NTHi分离株和未来的监测这些基因是必要的。
Objectives: To investigate the phenotypic effect of expression of selected acquired macrolide resistance genes (AMRGs) in non-typeable Haemophilus influenzae (NTHi).Methods: The AMRGs erm(A), erm(B) and erm(C) were cloned into Escherichia coli JM109 using the shuttle vector pLS88; constructed plasmids extracted from suitable clones were used to transform H. influenzae Rd by electroporation. Erythromycin and azithromycin MICs for suitable transformants were determined by broth microdilution. AMRG expression was determined using quantitative PCR on transformant cDNA with locked nucleic acid dual-labelled hydrolysis probes.Results: Expression of all AMRGs was observed in the transformants. Some variation in expression between the AMRGs was apparent, but expression of all genes was associated with a notable increase in erythromycin and azithromycin MICs compared with untransformed H. influenzae Rd.Conclusions: While the establishment of erm genes within WT populations of NTHi remains contentious, H. influenzae is capable of expression of erm. Expression may be associated with a subsequent decreased susceptibility to macrolides in isolates and future monitoring of these genes in NTHi isolates is warranted.