Expression of calcium-binding proteins S100A2 and S100A4 in Barrett's adenocarcinomas
Expression of calcium-binding proteins S100A2 and S100A4 in Barrett's adenocarcinomas
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DOI：
10.1593/neo.06481
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发表时间：
2006-10-01
期刊：
影响因子：
4.8
通讯作者：
El-Rifai, Wael
中科院分区：
文献类型：
--
作者：
Lee, Ok-Jae;Hong, Seung-Mo;El-Rifai, Wael
在这项研究中，我们研究了S100 A2和S100 A4在胃和食管腺癌中的mRNA和蛋白表达。对72例肿瘤的实时逆转录聚合酶反应分析显示，S100 A2和S100 A4在Barrett腺癌（BA）中频繁过表达（P <0.01）。免疫组化分析肿瘤组织微阵列，其中包含187个肿瘤显示缺乏到弱染色S100 A2在所有正常胃粘膜样本，而正常食管粘膜样本表现出中度到强烈的核染色。与正常食管粘膜中S100 A2的核表达相反，三分之二的Barrett不典型增生和过表达S100 A2的BA表现出比核染色更强的胞质染色（P < .001）。S100 A2蛋白的过度表达在高分化肿瘤中比在其他肿瘤中更常见（P = .02）。在三分之二的肿瘤中检测到S100 A4的中度至强染色，并且在存在Barrett食管的情况下经常观察到（P = .02）。与S100 A2相似，S100 A4在三分之二的肿瘤中主要表达于胞浆（P = 0.001）。S100 A4过表达与淋巴结转移显著相关（N-2-N-4）（P = 0.027）。这些结果表明在BA中S100 A2和S100 A4的频繁胞质过表达。进一步的研究正在进行中，以了解这些S100 A蛋白在巴雷特肿瘤发生中的生物学意义。
In this study, we investigated the mRNA and protein expression of S100A2 and S100A4 in adenocarcinomas of the stomach and esophagus. Real-time reverse transcription-polymerase reaction analysis on 72 tumors revealed frequent overexpression of S100A2 and S100A4 in Barrett's adenocarcinomas ( BAs) ( P < .01). Immunohistochemical analysis on tumor tissue microarrays that contained 187 tumors showed absent to weak staining for S100A2 in all normal gastric mucosa samples, whereas normal esophageal mucosa samples demonstrated moderate to strong nuclear staining. Contrary to the nuclear expression of S100A2 in normal esophageal mucosa, two thirds of Barrett's dysplasia and BAs that overexpressed S100A2 demonstrated stronger cytosolic staining than nuclear staining ( P < .001). Overexpression of S100A2 protein was more frequently seen in well-differentiated tumors than in others ( P = .02). Moderate to strong staining of S100A4 was detected in two thirds of tumors and was frequently observed in the presence of Barrett's esophagus ( P = .02). Similar to S100A2, the expression of S100A4 was predominantly cytosolic in two thirds of the tumors ( P = .001). There was a significant correlation between S100A4 overexpression and lymph node metastasis ( N-2-N-4) ( P = .027). These results demonstrate frequent cytosolic overexpression of S100A2 and S100A4 in BAs. Further studies are ongoing to understand the biological significance of these S100A proteins in Barrett's tumorigenesis.