COMPARATIVE ELECTROPHYSIOLOGY OF PYRAMIDAL AND SPARSELY SPINY STELLATE NEURONS OF THE NEOCORTEX
COMPARATIVE ELECTROPHYSIOLOGY OF PYRAMIDAL AND SPARSELY SPINY STELLATE NEURONS OF THE NEOCORTEX
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DOI：
10.1152/jn.1985.54.4.782
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
PRINCE, DA
中科院分区：
文献类型：
--
作者：
MCCORMICK, DA;CONNORS, BW;PRINCE, DA
将豚鼠感觉运动皮层和前扣带皮层切片保存在体外，并浸泡在正常生理培养基中。采用细胞内记录技术评价神经元电生理特性。细胞内注射荧光染料路西法黄CH对部分神经元进行形态学鉴定。观察到三种不同类型的神经元的电生理行为；它们被称为常规尖峰、爆裂尖峰和快速尖峰。感觉运动区和前扣带区神经元的生理特性无显著差异。正常尖峰细胞的动作电位在半振幅下平均持续时间为0.80 ms，尖峰最大上升速率与最大下降速率之比为4.12，并且在一系列尖峰后存在明显的后超极化现象。初始尖峰频率与注入电流强度的主要斜率为241 Hz/nA。在长时间的超阈电流脉冲中，放电频率具有很强的适应性。当局部突触通路被激活时，所有细胞都短暂兴奋，然后强烈抑制。爆发细胞的特点是它们能够产生内源性的、全有或无的3到5个动作电位的爆发。在其他方面，它们的特性与常规尖峰细胞非常相似。当膜去极化到接近强压电流的放电阈值时，产生脉冲的能力被消除。相比之下，常规尖峰（即非破裂）细胞的超极化并没有发现潜在的破裂倾向。快速尖峰细胞的动作电位比其他类型的细胞短得多（平均0.32 ms）。（摘要删节250字）
Slices of sensorimotor and anterior cingulate cortex from guinea pigs were maintained in vitro and bathed in a normal physiological medium. Electrophysiological properties of neurons were assessed with intracellular recording techniques. Some neurons were identified morphologically by intracellular injection of the fluorescent dye Lucifer yellow CH. Three distinct neuronal classes of electrophysiological behavior were observed; these were termed regular spiking, bursting, and fast spiking. The physiological properties of neurons from sensorimotor and anterior cingulate areas did not differ significantly. Regular-spiking cells were characterized by action potentials with a mean duration of 0.80 ms at one-half amplitude, a ratio of maximum rate of spike rise to maximum rate of fall of 4.12, and a prominent afterhyperpolarization following a train of spikes. The primary slope of initial spike frequency versus injected current intensity was 241 Hz/nA. During prolonged suprathreshold current pulses the frequency of firing adapted strongly. When local synaptic pathways were activated, all cells were transiently excited and then strongly inhibited. Bursting cells were distinguished by their ability to generate endogenous, all-or-none bursts of three to five action potentials. Their properties were otherwise very similar to regular-spiking cells. The ability to generate a burst was eliminated when the membrane was depolarized to near the firing threshold with tonic current. By contrast, hyperpolarization of regular-spiking (i.e., nonbursting) cells did not uncover latent bursting tendencies. The action potentials of fast-spiking cells were much briefer (mean of 0.32 ms) than those of the other cell types.(ABSTRACT TRUNCATED AT 250 WORDS)