Role of endothelin-1 receptors in the sarcolemma membrane and the nuclear membrane in the modulation of basal cytosolic and nuclear calcium levels in heart cells.
Role of endothelin-1 receptors in the sarcolemma membrane and the nuclear membrane in the modulation of basal cytosolic and nuclear calcium levels in heart cells.
复制标题
肌膜膜和核膜中的内皮素 1 受体在调节心脏细胞基础胞质和核钙水平中的作用。
作者：
G. Bkaily;D. Massaad;S. Choufani;D. Jacques;P. D'Orléans
我们先前在心脏细胞中的工作表明，核膜具有受体，例如血管紧张素II的受体。利用来自10天鸡胚心脏的非工作单细胞和共聚焦显微镜技术，我们目前的研究结果表明，刺激肌膜上的内皮素-1（ET-1）受体可诱导基础胞浆和核内钙水平的剂量依赖性持续增加，而这种增加对ET（A）和ET（B）受体拮抗剂BQ 123和BQ 788不敏感。胞浆中应用ET-1可诱导持续的基底核Ca（2+）浓度呈剂量依赖性增加，而这种增加对BQ 123和BQ 788不敏感。心肌细胞核膜上的ET-1受体对ET-1的敏感性高于肌膜上的受体。胞浆内应用ET-1可阻止细胞核持续的基础胞浆Ca（2+）摄取。ET-1荧光探针的使用表明，在肌膜膜和核膜上都存在ET-1受体。因此，我们的研究结果表明，ET-1受体是不敏感的BQ 123和BQ 788存在于肌膜和核膜。细胞外和胞浆ET-1可能对心肌细胞胞浆和核内基础Ca（2+）水平起调节作用。
Our previous work in heart cells showed that the nuclear envelope membranes possess receptors, such as those for angiotensin II. Using non-working single cells from the hearts of 10-day chick embryos and the confocal microscopy technique, our present results show that stimulation of endothelin-1 (ET-1) receptors at the sarcolemma membrane induced a dose-dependent sustained increase in basal cytosolic and nuclear calcium levels that was insensitive to the ET(A) and ET(B) receptor antagonists BQ123 and BQ788. The cytosolic application of ET-1 induced a dose-dependent increase in the sustained basal nuclear Ca(2+) concentration that was insensitive to BQ123 and BQ788. The ET-1 receptors at the nuclear envelope membrane were more sensitive to ET-1 than those located at the sarcolemma membrane of heart cells. Cytosolic application of ET-1 prevented sustained basal cytosolic Ca(2+) uptake by the nuclei. The use of an ET-1 fluorescent probe demonstrated the presence of ET-1 receptors at both the sarcolemma membrane and the nuclear envelope membrane. Thus our results suggest that ET-1 receptors that are insensitive to BQ123 and BQ788 are present at both the sarcolemma and nuclear envelope membranes. Extracellular and cytosolic ET-1 may play a role in regulating basal cytosolic and nuclear Ca(2+) levels in heart cells.
肝细胞核上的血管紧张素-II 结合位点。
影响因子：
4.8
作者：
Booz,GW;Conrad,KM;Hess,AL;Singer,HA;Baker,KM
通讯作者：
Baker,KM