Cytomegalovirus infection of the BS-1 human stroma cell line: effect on murine hemopoiesis.
Cytomegalovirus infection of the BS-1 human stroma cell line: effect on murine hemopoiesis.
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BS-1 人基质细胞系的巨细胞病毒感染：对小鼠造血的影响。
DOI：
10.1006/viro.1993.1498
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Chatis,PA
中科院分区：
文献类型：
--
作者：
Steinberg,HN;AndersonJr,J;Lim,B;Chatis,PA
BS-1是一种来源于人骨髓的基质细胞系，在短期体外培养体系中可支持小鼠红系(BFU-E)、粒-巨噬细胞(CFU-GM)和巨核系祖细胞(CFU-M)的生长。在共培养前将BS-1细胞暴露在巨细胞病毒(CMV)中3小时会导致基质细胞支持小鼠造血的能力显著降低。CMV对BS-1细胞造血支持功能的影响依赖于感染的多样性，在病毒与骨髓细胞1：1的比例下观察到BFU-E的完全抑制。以0.1：1的比例观察到BS-1细胞支持BFU-E的能力损失了50%。进一步对数稀释后，未观察到CMV的影响。巨细胞病毒感染BS-1细胞对其支持红系祖细胞生长的影响大于对粒细胞巨噬细胞集落形成的影响。CMV抗体或病毒热灭活可逆转对BS-1细胞的抑制作用。结果表明，巨细胞病毒可以感染构成正常骨髓微环境的细胞成分之一的细胞，导致基质支持正常祖细胞生长和发育的能力下降。
BS-1, a stromal cell line derived from human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-M) progenitor cells in a short termin vitrococulture system. Exposure of BS-1 cells to cytomegalovirus (CMV) for 3 hr prior to coculture results in a marked reduction in the stroma cell's ability to support murine hemopoiesis. CMV's effect on the BS-1 cell's hematopoietic support function is dependent on the multiplicity of infection with total suppression of BFU-E observed at a 1:1 ratio of virus to bone marrow cells. A 50% loss in the ability of BS-1 cells to support BFU-E is observed at a 0.1:1 ratio. No effect of CMV is observed with further log dilutions of virus. CMV infection of BS-1 cells affects its support of erythroid progenitor cell growth to a greater extent than its influence on the development of granulocyte-maccrophage colonies. Antibody to CMV or heat inactivation of the virus reverses the inhibitory affect on BS-1 cells. The results suggest that CMV can infect a cell that constitutes one of the cellular elements of the normal bone marrow microenvironment causing a decrease in the stroma's ability to support the growth and development of normal progenitor cells.