Vascular changes in the cerebral cortex in HIV-1 infection .2. An immunohistochemical and lectinhistochemical investigation
Vascular changes in the cerebral cortex in HIV-1 infection .2. An immunohistochemical and lectinhistochemical investigation
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DOI：
10.1007/s004010050486
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发表时间：
1996-07-01
影响因子：
12.7
通讯作者：
Weis, S
中科院分区：
文献类型：
--
作者：
Buttner, A;Mehraein, P;Weis, S
在人类免疫缺陷病毒1型（H1 V-1）感染的患者中，通过SPECT分析在不同脑区观察到低灌注，但尚未发现特定脑区占优势的特定模式。采用免疫组织化学和凝集素组织化学方法对获得性免疫缺陷综合征（AIDS）患者和正常对照组的大脑皮质、枕叶进行了分析。对IV型胶原、层粘连蛋白（基底层）和VIII因子（内皮细胞）进行免疫组化，并使用凝集素组织化学[蓖麻凝集素（RCA-I）、荆豆凝集素（UEA-I）、小麦胚芽凝集素（WGA）和大豆凝集素（SBA）]研究内皮细胞膜中糖蛋白的变化。在灰色和白色物质中对血管进行计数，并使用三点量表对不同抗体和凝集素的染色强度进行评级。艾滋病患者脑中IV型胶原蛋白的免疫反应性降低，这可能与脑血管基底层变薄有关，正如先前电子显微镜所示。用SEA、UEA-I和WGA进行的凝集素组织化学显示内皮细胞膜中的糖蛋白丢失。本研究的数据显示了艾滋病患者大脑中内皮细胞和基底膜的形态学变化，可能代表血脑屏障紊乱的形态学后遗症，或者可能解释了SPECT分析中观察到的灌注不足。
In human immunodeficiency virus 1 (H1V-1)-infected patients, a hypoperfusion is seen by SPECT analy ses in different brain regions but a specific pattern for the predominance of a specific brain region has not been found. The vessels of the cerebral cortex of the frontal, temporal, parietal, and occipital lobes of acquired immunodeficiency syndrome (AIDS) brains and control brains were analyzed by immunohistochemistry and lectin histochemistry. Immunohistochemistry was performed for collagen IV, laminin (basal lamina), and factor VIII (endothelial cell) and lectin histochemistry [Ricinus communis agglutinin (RCA-I), Ulex europaeus agglutinin (UEA-I), wheatgerm agglutinin (WGA) and soybean agglutinin (SBA)] was used to study changes of glycoproteins in the endothelial cell membrane. Vessels were counted in the gray and white matter, and their staining intensity for the different antibodies and lectins was rated using a three-point scale. Immunoreactivity for collagen IV was reduced in AIDS brains, which may be related to thinning of the basal lamina of cerebral vessels, as has previously been shown by electron microscopy. Lectin histochemistry with SEA, UEA-I and WGA indicated loss of glycoproteins in the membrane of endothelial cells. The data from the present study show morphological changes of the endothelial cells and of the basal lamina in the brain of individuals with AIDS, and might represent the morphological sequelae of a disturbed blood-brain barrier, or may account for the hypoperfusion seen in SPECT analyses.