CKMT1 and NCOA1 expression as a predictor of clinical outcome in patients with advanced-stage head and neck squamous cell carcinoma
CKMT1 and NCOA1 expression as a predictor of clinical outcome in patients with advanced-stage head and neck squamous cell carcinoma
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DOI：
10.1002/hed.24232
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Mangues, Ramon
中科院分区：
文献类型：
--
作者：
Angel Pavon, Miguel;Parreno, Matilde;Mangues, Ramon
背景。我们研究了头颈癌患者中一组先前确定的基因的表达与临床预后之间的关系。我们采用逆转录聚合酶链反应（RT-PCR）分析了III期至IVa/b化疗患者肿瘤活检组织中89个基因的表达（n=46）。通过RNAseq （The Cancer Genome Atlas [TCGA] project, n=371）或免疫组化（immunohistochemistry, n=73）分析另外两个队列来验证结果。30个基因与局部复发或无进展生存相关。预测局部复发的最佳多基因决策树模型包括核受体辅助激活因子1 （NCOA1）和血清淀粉样蛋白A2 （SAA2）表达，而预测疾病复发的最佳模型包括肌酸激酶线粒体1 （CKMT1）和金属调节转录因子1 （MTF1）。这两种模型都与癌症特异性生存有关。结果通过分析TCGA项目中包含的RNAseq数据得到证实。在免疫组织化学分析的独立队列中，CKMT1和NCOA1被确定为独立的生存危险因素。CKMT1和NCOA1表达在晚期头颈部癌中具有预后意义。(C) 2015 Wiley期刊公司
Background. We studied the association between the expression of a subset of previously identified genes and clinical outcome in patients with head and neck cancer.Methods. We analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) the expression of 89 genes in tumor biopsies from stage III to IVa/b chemotherapy treated patients (n=46). Two additional cohorts analyzed by RNAseq (The Cancer Genome Atlas [TCGA] project; n=371) or immunohistochemistry (IHC; n=73) were used to validate results.Results. Thirty genes were associated with local-recurrence or progression-free survival. The best multi-gene decision-tree model to predict local recurrence included nuclear receptor coactivator 1 (NCOA1) and serum-amyloid A2 (SAA2) expression, whereas the best model to predict disease recurrence included creatine kinase mitochondrial 1 (CKMT1) and metal-regulatory transcription factor 1 (MTF1). Both models were associated with cancer-specific survival. Results were confirmed analyzing the RNAseq data included in the TCGA project. CKMT1 and NCOA1 were identified as independent risk factors for survival in an independent cohort analyzed by immunohistochemistry.Conclusion. CKMT1 and NCOA1 expression has prognostic significance in advanced-stage head and neck carcinoma. (C) 2015 Wiley Periodicals, Inc.