SEQUENCE OF THE REGION CODING FOR VIRION PROTEINS C AND E2 AND THE CARBOXY TERMINUS OF THE NONSTRUCTURAL PROTEINS OF RUBELLA-VIRUS - COMPARISON WITH ALPHAVIRUSES
SEQUENCE OF THE REGION CODING FOR VIRION PROTEINS C AND E2 AND THE CARBOXY TERMINUS OF THE NONSTRUCTURAL PROTEINS OF RUBELLA-VIRUS - COMPARISON WITH ALPHAVIRUSES
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DOI：
10.1016/0378-1119(88)90582-3
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发表时间：
1988-01-01
期刊：
影响因子：
3.5
通讯作者：
MARR, LD
中科院分区：
文献类型：
--
作者：
FREY, TK;MARR, LD
测定了风疹病毒（RV）Therein株基因组RNA的3“4508个核苷酸（nt）的cDNA克隆序列。该序列包含一个3189-nt的开放阅读框（ORF），其编码结构蛋白C、E2和E1。预测C具有300个氨基酸的长度（a）。C蛋白的N-末端一半是高度碱性和亲水性的，并且是与病毒体RNA相互作用的蛋白质区域。在C蛋白的C末端是一段由20个疏水性aa组成的序列，它也充当E2的信号序列，这表明C从多聚蛋白前体中的切割可能是由内质网腔中的信号酶催化的。E2全长282个氨基酸，在C端含有4个潜在的N-糖基化位点和一个跨膜结构域。先前已经描述了E1的序列[Frey等人，Virology 154（1986）228-232]。RV结构蛋白的氨基酸序列与甲病毒结构蛋白的氨基酸序列之间没有同源性。从RV基因组序列中与甲病毒基因组中亚基因组RNA合成起始的序列显示出显著同源性的30 nt区域的位置，预测RV亚基因组RNA的长度为3346 nt，并且预测从亚基因组RNA的5“末端到结构蛋白ORF的非翻译区为98 nt。在一个不同的翻译框架中，从这里报道的RV nt序列的5“末端开始，是一个1407 nt的ORF，它是非结构蛋白ORF的C末端区域。该ORF与结构蛋白ORF重叠149 nt。RV非结构蛋白ORF C-末端的预测氨基酸序列与动物和植物的几种正极性RNA病毒的复制酶蛋白之间可以检测到低水平的同源性，包括甲病毒的nsp 4，由甲病毒非结构ORF C-末端区域编码的蛋白。然而，RV和甲病毒在该基因组区域的总体同源性仅为18%，表明披膜病毒科的这两个属仅是远亲。有趣的是，在RV序列的负极性方向上存在一个2844-nt的ORF，其可以编码928-aa的多聚蛋白。
The sequence of the 3'' 4508 nucleotides (nt) of the genomic RNA of the Therein strain of rubella virus (RV) was determined for cDNA clones. The sequence contains a 3189-nt open reading frame (ORF) which codes for the structural proteins C, E2 and E1. C is predicted to have a lenght of 300 amino acids (a). The N-terminal half of the C protein is highly basic and hydrophilic in nature, and is putatively the region of the protein which interacts with the virion RNA. At the C terminus of the C protein is a stretch of 20 hydrophobic aa which also serves as the signal sequence for E2, indicating that the cleavage of C from the polyprotein precursor may be catalyzed by signalase in the lumen of the endoplasmic reticulum. E2 is 282 aa in length and contains four potential N-linked glycosylation sites and putative transmembrane domain near its C terminus. The sequence of E1 has been previously described [Frey et al., Virology 154 (1986) 228-232]. No homology could be deteced between the amino acid sequence of the RV structural proteins and the amino acid sequence of the alphavirus structural proteins. From the position of a region of 30 nt in the RV genomic sequence which exhibited significant homology with the sequence in the alphavirus genome at which subenomic RNA synthesis is initiated, the RV subgenomic RNA is prediced to be 3346 nt in length and the nontranslated region from the 5'' end of the subgenomic RNA to the structural protein ORF is predicted to be 98 nt. In a different translation frame beginning at the 5'' end of the RV nt sequence reported here is a 1407 nt ORF which is the C terminal region of the nonstructural protein ORF. This ORF overlaps the structural protein ORF by 149 nt. A low level of homology could be detected between the predicted amino acid sequence of the C-terminus of the RV nonstructural protein ORF and the replicase proteins of several positive polarity RNA viruses of animals and plants, including nsp4 of the alphaviruses, the protein encoded by the C-terminal region of the alphavirus nonstructural ORF. However, the overall homology between RV and the alphaviruses in this region of the genome was only 18%, indicating that these two genera ofd the Togavirus family are only distantly related. Intriguingly, there is a 2844-nt ORF present in the negative polarity orientation of the RV sequence which could encode a 928-aa polyprotein.