ALKALINE-PHOSPHATASE INDUCES THE MINERALIZATION OF SHEETS OF COLLAGEN IMPLANTED SUBCUTANEOUSLY IN THE RAT
ALKALINE-PHOSPHATASE INDUCES THE MINERALIZATION OF SHEETS OF COLLAGEN IMPLANTED SUBCUTANEOUSLY IN THE RAT
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DOI：
10.1172/jci115805
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发表时间：
1992-06-01
影响因子：
15.9
通讯作者：
VANDENBOS, T
中科院分区：
文献类型：
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作者：
BEERTSEN, W;VANDENBOS, T
为了确定碱性磷酸酶（ALP）是否可以在体内引起胶原基质的矿化，牛肠ALP共价结合到胍提取的脱矿牛牙本质（DDS）切片。将制备物皮下植入大鼠颅骨的右半部分。将未用酶处理的对照切片植入相同动物头骨的左半部分。标本收获后的时期从1到4周不等。结果表明，ALP偶联的DDS快速积累羟基磷灰石晶体。植入4周后，每微克羟脯氨酸中钙和磷酸盐的含量分别达到正常牛牙本质的80%和60%。我们的观察提供了直接的证据表明，ALP可能在体内胶原基质中诱导羟基磷灰石沉积中起着至关重要的作用。
To determine whether alkaline phosphatase (ALP) can cause the mineralization of collagenous matrices in vivo, bovine intestinal ALP was covalently bound to slices of guanidine-extracted demineralized bovine dentin (DDS). The preparations were implanted subcutaneously over the right half of the rat skull. Control slices not treated with the enzyme were implanted over the left half of the skull of the same animals. Specimens were harvested after periods varying from 1 to 4 wk. It was shown that ALP-coupled DDS rapidly accumulated hydroxyapatite crystals. 4 wk after implantation, the content of calcium and phosphate per Microgram of hydroxyproline amounted up to 80 and 60%, respectively, of that found in normal bovine dentin. Our observations present direct evidence that ALP may play a crucial role in the induction of hydroxyapatite deposition in collagenous matrices in vivo.