Antispermatogenic and hormonal effects of Crotalaria juncea Linn. seed extracts in male mice.
Antispermatogenic and hormonal effects of Crotalaria juncea Linn. seed extracts in male mice.
复制标题
猪屎豆的抗生精和激素作用。
DOI：
--
复制
发表时间：
2004
影响因子：
2.9
通讯作者：
S. B. Patil
中科院分区：
文献类型：
--
作者：
B. Vijaykumar;I. Sangamma;A. Sharanabasappa;S. B. Patil
目的
评估猪屎豆种子的各种提取物对雄性小鼠的抗生育活性。
方法
成年雄性小鼠灌胃芥菜种子石油醚、苯和乙醇提取物25mg×(100g)(-1)×天(-1)，持续30天。第31天，通过颈脱位处死动物，解剖出睾丸、附睾、输精管、精囊、前列腺、尿道球腺和提肛肌并称重。对器官进行处理以进行生化和组织学检查。
结果
在石油醚、苯和乙醇提取物治疗的大鼠中，睾丸和附属生殖器官的重量有所下降。睾丸和生精小管的直径减小。睾丸中的精原细胞、精母细胞和精细胞以及附睾尾部的精子数量也减少。睾丸、附睾和输精管中蛋白质和糖原含量显着减少，胆固醇含量增加。在 3 种提取物中，乙醇提取物似乎具有最有效的抗生精活性。当在未成熟的雄性小鼠中测试乙醇提取物时，由于附属器官的重量减少，因此具有抗雄激素作用。
结论
芥菜种子的各种提取物可抑制精子发生，并可能具有抗雄激素活性。
AIM
To evaluate the antifertility activity of various extracts of Crotalaria juncea seeds in male mice.
METHODS
Adult male mice were gavaged the petroleum ether, benzene and ethanol extracts of C. juncea seeds, 25 mg x (100g)(-1) x day(-1) for 30 days. On day 31 the animals were sacrificed by cervical dislocation and the testes, epididymis, vas deferens, seminal vesicles, prostate gland, bulbourethral gland and levator ani were dissected out and weighed. The organs were processed for biochemical and histological examination.
RESULTS
In petroleum ether, benzene and ethanol extracts treated rats, there was a decrease in the weights of testis and accessory reproductive organs. The diameters of the testis and seminiferous tubules were decreased. Spermatogonia, spermatocytes and spermatids in the testis and the sperm count in cauda epididymis were also decreased. There was a significant reduction in the protein and glycogen contents and an increase in the cholesterol content in the testis, epididymis and vas deferens. Of the 3 extracts, the ethanol extract appeared to be the most potent in antispermatogenic activity. When the ethanol extract was tested in immature male mice, there was an antiandrogenic effect as the weights of accessory organs were reduced.
CONCLUSION
The various extracts of C. juncea seeds arrest spermatogenesis and are likely to have an antiandrogenic activity.