Involvement of GATA-4/-5 transcription factors in ovarian carcinogenesis
Involvement of GATA-4/-5 transcription factors in ovarian carcinogenesis
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DOI：
10.1016/j.canlet.2005.10.039
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发表时间：
2006-09-28
期刊：
影响因子：
9.7
通讯作者：
Yuasa, Yasuhito
中科院分区：
文献类型：
--
作者：
Wakana, Kimio;Akiyama, Yoshimitsu;Yuasa, Yasuhito
为了阐明加塔转录因子在卵巢癌发生中的作用，我们分析了加塔-4/-5/-6在8个人卵巢癌细胞系中的表达和甲基化状态。加塔-4/-5分别在三个和两个不表达它们的细胞系中被甲基化。加塔-4/-5的甲基化也分别在15例原发性卵巢癌中的9例和5例中检测到。加塔-6在任何情况下都没有甲基化。我们使用腺病毒系统在JHOC-5细胞系中瞬时过表达加塔-5，导致诱导凋亡和凋亡相关基因，如Apaf-1，上调。提示加塔-4/-5可能参与卵巢癌的发生。(c)2006爱思唯尔爱尔兰有限公司保留所有战斗。
To clarify the role of GATA transcription factors in ovarian carcinogenesis, we analyzed the expression and methylation states of GATA-4/-5/-6 in eight human ovarian cancer cell lines. GATA-4/-5 were methylated in three and two cell lines without their expression, respectively. Methylation of GATA-4/-5 was also detected in nine and five of 15 primary ovarian cancers, respectively. GATA-6 was not methylated in any cases. We transiently over-expressed GATA-5 in the JHOC-5 cell line using an adenovirus system, resulting in that apoptosis was induced and apoptosis-related genes, such as Apaf-1, were up-regulated. These data suggest that GATA-4/-5 may be involved in ovarian carcinogenesis. (c) 2006 Elsevier Ireland Ltd. All fights reserved.