Polyclonal activation of human peripheral blood B lymphocytes by Fusobacterium nucleatum.
Polyclonal activation of human peripheral blood B lymphocytes by Fusobacterium nucleatum.
复制标题
具核梭杆菌对人外周血 B 淋巴细胞的多克隆激活。
DOI：
10.1128/iai.40.3.1104-1111.1983
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Lopatin,DE
中科院分区：
文献类型：
--
作者：
Mangan,DF;Lopatin,DE
本研究检测了核梭杆菌体外对人b淋巴细胞（b细胞）的激活作用。美洲商陆有丝分裂原是一种经过充分研究的PBA激活剂，被包括在一些实验中进行比较。通过微酶联免疫吸附法测定培养上清液中总免疫球蛋白A、G和M的浓度来测定PBA。在单核细胞缺失的培养物中，1到10微克/毫升的核心菌对PBA的刺激效果最佳，而在未分离的、含有单核细胞的培养物中，商陆有丝分裂原的反应效果最佳。免疫球蛋白合成主要发生在核仁梭菌刺激后的第6天至第8天。T淋巴细胞增强了PBA对具核梭菌的反应，特别是当T细胞与b细胞的比例为1:1时。淋巴细胞浓度为每孔200,000细胞时，圆底井的免疫球蛋白产量高于平底井。然而，在含有较高淋巴细胞浓度的平底孔中，PBA反应显著增加，这表明PBA通过细胞间接触而增强。核仁梭菌刺激淋巴细胞的延迟导致免疫球蛋白的产生减少。本研究结果为核仁梭菌对体外PBA的调控提供了依据。这些数据还表明，有核梭菌和美洲商陆有丝分裂原刺激PBA的机制可能存在差异。
The present study examined in vitro polyclonal human B-lymphocyte (B-cell) activation (PBA) by Fusobacterium nucleatum. Pokeweed mitogen, a well-studied PBA activator, was included in some experiments for comparison. PBA was determined by the total immunoglobulin A, G, and M concentrations in the culture supernatants as measured by micro-enzyme-linked immunosorbent assay. F. nucleatum, at concentrations between 1 and 10 micrograms/ml, stimulated optimal PBA in monocyte-depleted cultures, whereas the pokeweed mitogen response was optimal in unfractionated, monocyte-containing cultures. Immunoglobulin synthesis occurred primarily between days 6 and 8 after stimulation with F. nucleatum. T lymphocytes enhanced the PBA response to F. nucleatum, particularly at a T- to B-cell ratio of 1:1. Immunoglobulin production was greater in round-bottomed wells than in flat-bottomed wells at lymphocyte concentrations of 200,000 cells per well. The PBA response, however, increased dramatically in flat-bottomed wells containing higher lymphocyte concentrations, suggesting that PBA is enhanced by cell-to-cell contact. A delay in stimulation of the lymphocytes with F. nucleatum resulted in diminished immunoglobulin production. The results provide information on the regulation of in vitro PBA induced by F. nucleatum. The data also suggest that there may be differences in the mechanisms by which F. nucleatum and pokeweed mitogen stimulate PBA.