Increased mRNA levels of tyrosine hydroxylase and dopamine transporter in the VTA of male rats after chronic food restriction
Increased mRNA levels of tyrosine hydroxylase and dopamine transporter in the VTA of male rats after chronic food restriction
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DOI：
10.1111/j.1460-9568.2005.04531.x
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Schiöth, HB
中科院分区：
文献类型：
--
作者：
Lindblom, J;Johansson, A;Schiöth, HB
节食作为减轻体重的策略通常会失败，因为它会引起对食物的渴望，从而导致暴饮暴食和体重反弹。多项研究的证据表明，对食物和药物渴望的神经调节存在共同的元素。我们使用定量实时聚合酶链式反应，对长期食物限制的雄性大鼠中脑边缘和黑质纹状体多巴胺系统相关的大脑区域中参与多巴胺信号传导的八个基因的表达进行了定量。食物限制强烈增加了腹侧被盖区酪氨酸羟化酶和多巴胺转运蛋白的 mRNA 水平。定量放射自显影表明，伏隔核壳中多巴胺转运蛋白的蛋白质水平也上调。然而，在急性食物剥夺后并没有观察到这些影响。我们认为，结果反映了中脑边缘多巴胺通路的敏化，其特征是伏隔核壳中细胞外多巴胺的清除增加。中脑边缘多巴胺系统的这种敏感性可能是干扰饮食依从性的食物渴望的根本原因之一。
Dieting as a strategy to reduce body weight often fails as it causes food cravings leading to bingeing and weight regain. Evidence from several lines of research suggests the presence of shared elements for neural regulation of food and drug craving. We quantified the expression of eight genes involved in dopamine signalling in brain regions related to the mesolimbic and nigrostriatal dopamine system in male rats subjected to chronic food restriction using quantitative real-time polymerase chain reaction. Food restriction strongly increased mRNA levels of tyrosine hydroxylase and the dopamine transporter in the ventral tegmental area. Quantitative autoradiography indicated that the dopamine transporter was also upregulated at the protein level in the shell of the nucleus accumbens. However, these effects were not observed after acute food deprivation. We suggest that the results reflect a sensitization of the mesolimbic dopamine pathway characterized by increased clearance of extracellular dopamine in the nucleus accumbens shell. Such sensitization of the mesolimbic dopamine system may be one of the underlying causes for the food cravings that interfere with dietary compliance.