Differential DNA methylation in experienced meditators after an intensive day of mindfulness-based practice: Implications for immune-related pathways
Differential DNA methylation in experienced meditators after an intensive day of mindfulness-based practice: Implications for immune-related pathways
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DOI：
10.1016/j.bbi.2019.11.003
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发表时间：
2020-02-01
影响因子：
15.1
通讯作者：
Kaliman, P.
中科院分区：
文献类型：
--
作者：
Chaix, R.;Fagny, M.;Kaliman, P.
人类的甲基组受到心理压力的动态影响。然而，它对压力管理的反应仍未得到充分研究。冥想练习已被证明可以显著降低压力水平，以及其他有益的神经生理学结果。在这里，我们评估了一天的密集冥想练习(t(2)-t(1)=8h)对有经验的冥想者(n=17)外周血单核细胞甲基组的影响。同时，我们评估了在相同环境中一天的休闲活动对没有冥想经验的匹配对照组受试者(n=17)甲基组的影响。用Illumina 450K微珠芯片阵列分析DNA甲基化情况。我们为每个甲基化位点拟合了一个多水平实验的线性模型，该模型针对基线差异调整t(1)和t(2)之间的差异。两组间的甲基化分布没有发现显著的基线差异。在冥想组，我们在干预后确定了61个差异甲基化位点(DMS)。这些DM富含主要与免疫细胞新陈代谢和衰老相关的基因，以及参与免疫反应和炎症等功能的几种转录因子的结合位点。在对照组，休闲活动一天后，甲基化水平没有明显变化。这些结果表明，在训练有素的受试者中进行短暂的冥想干预，可能会迅速影响与免疫功能潜在相关的表观基因组，并在短时间窗口内更好地了解人类甲基组的动态。
The human methylome is dynamically influenced by psychological stress. However, its responsiveness to stress management remains underexplored. Meditation practice has been shown to significantly reduce stress level, among other beneficial neurophysiological outcomes. Here, we evaluated the impact of a day of intensive meditation practice (t(2)-t(1) = 8 h) on the methylome of peripheral blood mononuclear cells in experienced meditators (n = 17). In parallel, we assessed the influence of a day of leisure activities in the same environment on the methylome of matched control subjects with no meditation experience (n = 17). DNA methylation profiles were analyzed using the Illumina 450 K beadchip array. We fitted for each methylation site a linear model for multi-level experiments which adjusts the variation between t(1) and t(2) for baseline differences. No significant baseline differences in methylation profiles was detected between groups. In the meditation group, we identified 61 differentially methylated sites (DMS) after the intervention. These DMS were enriched in genes mostly associated with immune cell metabolism and ageing and in binding sites for several transcription factors involved in immune response and inflammation, among other functions. In the control group, no significant change in methylation level was observed after the day of leisure activities. These results suggest that a short meditation intervention in trained subjects may rapidly influence the epigenome at sites of potential relevance for immune function and provide a better understanding of the dynamics of the human methylome over short time windows.