Development of a biodegradable iron oxide nanoparticle gel for tumor bed therapy.
Development of a biodegradable iron oxide nanoparticle gel for tumor bed therapy.
复制标题
开发用于肿瘤床治疗的可生物降解的氧化铁纳米颗粒凝胶。
DOI：
10.1117/12.2007310
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hoopes,Pj
中科院分区：
文献类型：
--
作者：
Cunkelman,Bp;Chen,Ey;Petryk,Aa;Tate,Ja;Thappa,Sg;Collier,Rj;Hoopes,Pj
由于大多数肿瘤切除后复发发生在肿瘤边缘，术后手术床的治疗已被证明是有吸引力的。一种新型的可生物降解的普鲁兰凝胶注入磁性氧化铁纳米颗粒（IONP），用于手术床上给药，然后通过交变磁场（AMF）激活进行热疗。pulullan是一种水溶性成膜淀粉聚合物，可在术后伤口部位降解，递送IONP有效载荷，靶向剩余的癌细胞。不同的凝胶配方含有不同% wt的普鲁兰进行IONP洗脱试验。通过将凝胶浸泡在去离子水中1小时，然后测量上清液中的颗粒浓度和剩余凝胶配方的质量，来测量洗脱水平和凝胶降解量。最有希望的凝胶配方将在手术床切除的小鼠模型中进行测试，以评估体内凝胶溶解，通过组织学和透射电镜分析的离子p细胞摄取动力学，以及凝胶在AMF暴露下的加热能力。
Treatments of the post-operative surgical bed have proven appealing as the majority of cancer recurrence following tumor resection occurs at the tumor margin. A novel, biodegradable pullulan-based gel infused with magnetic iron oxide nanoparticles (IONP) is presented here for surgical bed administration followed by hyperthermia therapy via alternating magnetic field (AMF) activation. Pullulan is a water soluble, film-forming starch polymer that degrades at the postoperative wound site to deliver the IONP payload, targeting the remaining cancer cells. Different gel formulations containing various % wt of pullulan were tested for IONP elution. Elution levels and amount of gel degradation were measured by immersing the gel in de-ionized water for one hour then measuring particle concentrations in the supernatant and the mass of the remaining gel formulation. The most promising gel formulations will be tested in a murine model of surgical bed resection to assess in vivo gel dissolution, IONP cell uptake kinetics via histology and TEM analysis, and heating capability of the gel with AMF exposure.