Dietary L-threonine supplementation attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage of broiler chickens at an early age
Dietary L-threonine supplementation attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage of broiler chickens at an early age
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DOI：
10.1017/s0007114518000740
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发表时间：
2018-06-14
影响因子：
3.6
通讯作者：
Zhou, Yanmin
中科院分区：
文献类型：
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作者：
Chen, Yueping;Zhang, Hao;Zhou, Yanmin
本试验旨在研究饲粮中添加l -苏氨酸（L-Thr）对脂多糖（LPS）刺激下肉鸡生长性能、炎症反应和肠道屏障功能的保护作用。选取144只1日龄雄性雏鸡，分为3个处理：未攻毒肉鸡饲喂基础饲粮（对照组）、lps攻毒肉鸡饲喂基础饲粮中不添加l -苏氨酸和lps攻毒肉鸡饲喂基础饲粮中添加3.0 g/kg l -苏氨酸。分别于17、19、21日龄腹腔注射LPS，对照组注射生理盐水。与对照组相比，LPS刺激降低了肉仔鸡的生长性能，l -苏氨酸可逆转LPS诱导的料重比升高。LPS刺激与炎症相关的血细胞计数升高，以及血清（IL-1 β和tnf - α）、脾脏（IL-1 β和tnf - α）和肠黏膜（空肠干扰素- γ (ifn - γ)和回肠IL-1 β）中促炎细胞因子浓度升高。添加l -苏氨酸可降低lps攻击肉鸡肠道细胞因子浓度。LPS增加了循环d -乳酸浓度，降低了空肠和回肠的绒毛高度、绒毛高度与隐窝深度之比和杯状密度。添加l -苏氨酸后，lps诱导的空肠绒毛高度、肠绒毛高度/隐窝深度比和回肠杯状细胞密度的降低与添加l -苏氨酸后相反。同样，lps诱导的肠道炎症和屏障功能相关基因（空肠toll样受体4、ifn - γ和cladin -3、回肠IL-1 β和闭塞带-1）的肠道mRNA丰度的改变在给予L-Thr后恢复正常。由此可见，饲粮中添加l -苏氨酸可减轻脂多糖诱导的炎症反应和肠屏障损伤。
This study was conducted to investigate the protective effects of L-threonine (L-Thr) supplementation on growth performance, inflammatory responses and intestinal barrier function of young broilers challenged with lipopolysaccharide (LPS). A total of 144 1-d-old male chicks were allocated to one of three treatments: non-challenged broilers fed a basal diet (control group), LPS-challenged broilers fed a basal diet without L-Thr supplementation and LPS-challenged broilers fed a basal diet supplemented with 3.0 g/kg L-Thr. LPS challenge was performed intraperitoneally at 17, 19 and 21 d of age, whereas the control group received physiological saline injection. Compared with the control group, LPS challenge impaired growth performance of broilers, and L-Thr administration reversed LPS-induced increase in feed/gain ratio. LPS challenge elevated blood cell counts related to inflammation, and pro-inflammatory cytokine concentrations in serum (IL-1 beta and TNF-alpha), spleen (IL-1 beta and TNF-alpha) and intestinal mucosa (jejunal interferon-gamma (IFN-gamma) and ileal IL-1 beta). The concentrations of intestinal cytokines in LPS-challenged broilers were reduced by L-Thr supplementation. LPS administration increased circulating D-lactic acid concentration, whereas it reduced villus height, the ratio between villus height and crypt depth and goblet density in both jejunum and ileum. LPS-induced decreases in jejunal villus height, intestinal villus height: crypt depth ratio and ileal goblet cell density were reversed with L-Thr supplementation. Similarly, LPS-induced alterations in the intestinal mRNA abundances of genes related to intestinal inflammation and barrier function (jejunal toll-like receptor 4, IFN-gamma and claudin-3, and ileal IL-1 beta and zonula occludens-1) were normalised with L-Thr administration. It can be concluded that L-Thr supplementation could attenuate LPS-induced inflammatory responses and intestinal barrier damage of young broilers.