Shifting foci of hematopoiesis during reconstitution from single stem cells
Shifting foci of hematopoiesis during reconstitution from single stem cells
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DOI：
10.1073/pnas.2637010100
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发表时间：
2004-01-06
影响因子：
11.1
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Cao, YA;Wagers, AJ;Contag, CH
为了实时揭示活体动物造血重建的早期事件和动力学，我们使用生物发光成像来监测辐射受体中单核苷酸酶标记的造血干细胞（HSC）的植入。移植的HSC在脾和骨髓（BM）中产生离散病灶，其频率与BM隔室大小相关。最初检测到的病灶可以局部扩大，在BM或脾脏中的其他部位播种，和/或以不同的动力学消退。这些研究揭示了高度纯化的HSC的动态和可变的植入模式，并表明个体HSC对造血嵌合体的最终总体贡献不取决于初始植入和扩增的特定位点。
To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the spleen and bone marrow (BM), at a frequency that correlated with BM compartment size. Initially detected foci could expand locally, seed other sites in BM or spleen, and/or recede with different kinetics. These studies reveal dynamic and variable patterns of engraftment from highly purified HSC and indicate that the final overall contribution of individual HSC to hematopoietic chimerism does not depend on the specific site of initial engraftment and expansion.