Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster
Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster
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DOI：
10.1002/cne.23955
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发表时间：
2016-04-15
影响因子：
2.5
通讯作者：
Kamikouchi, Azusa
中科院分区：
文献类型：
--
作者：
Matsuo, Eriko;Seki, Haruyoshi;Kamikouchi, Azusa
昆虫间的声学通讯是分析听觉信息处理潜在神经机制的绝佳模型系统。大脑中听觉神经回路的详细结构尚未得到描述。为了解中枢听觉通路，我们以果蝇（Drosophila melanogaster）的大脑为模型，对与初级听觉中心相关的中间神经元进行了大规模分析。通过筛选表达驱动株系并对这些株系进行单细胞标记，我们鉴定出44种支配初级听觉中心的中间神经元。其中5种是局部中间神经元，而另外39种是将初级听觉中心与大脑其他区域相连的投射中间神经元。投射神经元包含三条频率选择性通路和两条频率包容性通路。对其连接靶点的映射显示，大脑中的五个神经纤维网——楔形体（WED）、前腹外侧原脑、后腹外侧原脑（PVLP）、鞍形体（SAD）和颚神经节（GNG）——与初级听觉中心紧密相连。此外，大脑中的其他几个神经纤维网，包括视觉和嗅觉中心，也直接与初级听觉中心相连。已鉴定神经元的棘突和突触小体的分布模式表明，听觉信息主要从初级听觉中心传递至PVLP、WED、SAD、GNG以及胸腹神经节。基于这些发现，我们绘制出首张次级听觉中间神经元综合图谱，该图谱揭示了信息向下游并行上升通路、多模态通路和下行通路的流动情况。（c）2016 威利期刊公司
Acoustic communication between insects serves as an excellent model system for analyzing the neuronal mechanisms underlying auditory information processing. The detailed organization of auditory neural circuits in the brain has not yet been described. To understand the central auditory pathways, we used the brain of the fruit fly Drosophila melanogaster as a model and performed a large-scale analysis of the interneurons associated with the primary auditory center. By screening expression driver strains and performing single-cell labeling of these strains, we identified 44 types of interneurons innervating the primary auditory center. Five types were local interneurons whereas the other 39 types were projection interneurons connecting the primary auditory center with other brain regions. The projection neurons comprised three frequency-selective pathways and two frequency-embracive pathways. Mapping of their connection targets revealed that five neuropils in the brainthe wedge (WED), anterior ventrolateral protocerebrum, posterior ventrolateral protocerebrum (PVLP), saddle (SAD), and gnathal ganglia (GNG)were intensively connected with the primary auditory center. In addition, several other neuropils, including visual and olfactory centers in the brain, were directly connected to the primary auditory center. The distribution patterns of the spines and boutons of the identified neurons suggest that auditory information is sent mainly from the primary auditory center to the PVLP, WED, SAD, GNG, and thoracico-abdominal ganglia. Based on these findings, we established the first comprehensive map of secondary auditory interneurons, which indicates the downstream information flow to parallel ascending pathways, multimodal pathways, and descending pathways. (c) 2016 Wiley Periodicals, Inc.