In vitro control of organogenesis and body patterning by activin during early amphibian development
In vitro control of organogenesis and body patterning by activin during early amphibian development
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DOI：
10.1016/s0305-0491(00)00195-4
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发表时间：
2000-06-01
影响因子：
2.2
通讯作者：
Malacinski, GM
中科院分区：
文献类型：
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作者：
Asashima, M;Ariizumi, T;Malacinski, GM
在两栖动物发育过程中，通过细胞分裂、基因顺序表达、形态发生和细胞分化等过程建立胚胎体计划。人体图案化的机制是复杂的，包括多个诱导事件。激活素是一种转化生长因子-β家族蛋白，可在动物帽子外植体中诱导多种中胚层和内胚层组织，并呈剂量依赖关系。在最近一项关于激活素在器官发生中的作用的研究中，我们通过用高浓度的激活素处理动物的帽子，成功地提高了心脏跳动。激活素还与维甲酸一起参与肾脏器官发生。活化素和维甲酸在体外诱导的胚胎肾脏，如果移植到已经去除前肾残骸的幼虫体内，就可以在体内发挥作用。此外，经过激活素处理的动物帽子清楚地显示了组织者的行动，这些行动与沿前后轴的身体模式密切相关。这些实验将有助于作为在细胞和分子水平上理解器官发生和身体模式的模型系统。(C)2000 Elsevier Science Inc.保留所有权利。
In the process of amphibian development, an embryonic body plan is established through cell division, sequential gene expression, morphogenesis and cell differentiation. The mechanism of body patterning is complex and includes multiple induction events. Activin, a TGF-beta family protein, can induce several kinds of mesodermal and endodermal tissues in animal cap explants in a dose-dependent manner. In a recent study of the role of activin in organogenesis, we succeeded in raising a beating heart by treating animal caps with a high concentration of activin. Activin also participates in kidney organogenesis in combination with retinoic acid. An embryonic kidney induced by activin and retinoic acid in vitro can function in vivo when it is transplanted into a larva in which pronephros rudiments have already been removed. Further, the activin-treated animal caps clearly show organizer actions that are closely related to body patterning along the anteroposterior axis. These experiments will help to serve as a model system for understanding organogenesis and body patterning at the cellular and molecular levels. (C) 2000 Elsevier Science Inc. All rights reserved.