Peptide bond synthesis:: Function of the efp gene product
Peptide bond synthesis:: Function of the efp gene product
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DOI：
10.1515/bc.2000.071
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发表时间：
2000-07-01
影响因子：
3.7
通讯作者：
Aoki, H
中科院分区：
文献类型：
--
作者：
Ganoza, MC;Aoki, H
efp基因编码的蛋白质是大肠杆菌细胞生长和生存所必需的。该基因的中断会导致细胞死亡，这是由于蛋白质合成的缺陷。我们在这里报告说，由efp基因编码的EFP蛋白是在由天然模板编程的系统中体外重建多肽合成所必需的，该模板包含每种纯化的起始因子IF 1、IF 2、IF 3;延伸因子，EFTu、EFT和EFG，以及一种称为W的蛋白质，它是从核糖体排出tRNA所需的。EFP蛋白是在所有上述因子存在下提高合成速率和程度所必需的。只有当N-乙酰基Phe-tRNA在促进70 S核糖体解离的条件下启动反应时，EFP蛋白才刺激用poly（rU）编程的poly（Phe）的合成。对核糖体从CCA氨基酰基受体合成许多fMet起始的二肽的能力的研究表明，EFP起到促进与受体合成的作用，所述受体是重建的肽基转移酶的不良供体。
The efp gene encodes a protein that is essential for the growth and for the viability of Escherichia coli cells. Interruption of this gene results in cell death due to a defect in protein synthesis. We report here that the EFP protein, encoded by the efp gene, is required for in vitro reconstitution of polypeptide synthesis in a system programmed by a native template which contains each of the purified initiation factors, IF1, IF2, IF3; the elongation factors, EFTu, EFTs and EFG, and a protein called W that is required to eject tRNAs from ribosomes. The EFP protein is required for enhancing the rate and the extent of synthesis in the presence of all of the above factors. The EFP protein stimulates synthesis of poly(Phe) programmed with poly(rU) only if N-acetyl Phe-tRNA initiates the reactions under conditions that foster the dissociation of the 70S ribosome. Study of the ability of the ribosome to synthesize a number of fMet-initiated dipeptides from CCA amino acyl accepters suggests that EFP acts to promote synthesis with accepters that are poor donors for the the reconstituted peptidyl transferase.