Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media.
Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media.
复制标题
在色氨酸结构化培养基中增强人多能干细胞增殖的方案。
DOI：
10.1016/j.xpro.2022.101341
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Tohyama, Shugo
中科院分区：
文献类型：
--
作者：
Kameda, Kotaro;Someya, Shota;Fujita, Jun;Fukuda, Keiichi;Tohyama, Shugo
我们描述了一种通过在常规培养基中补充L-色氨酸（TRP）来有效培养人多能干细胞（hPSC）的方案。TRP是一种必需氨基酸，其以负担得起的成本广泛可用，从而与单独使用常规培养基相比允许hPSC的成本有效的增殖。在这里，我们描述了用于增强来自真皮成纤维细胞或外周血细胞的hPSC增殖的步骤，但是该方案可以应用于任何hPSC。有关本方案使用和执行的完整详细信息，请参阅。从常规hPSC维持培养基制备TRP强化培养基用TRP强化培养基替代常规hPSC维持培养基在TRP强化培养基中培养的hPSC的增殖和多能性的评价我们描述了通过用L-色氨酸（TRP）补充常规培养基来有效培养人多能干细胞（hPSC）的方案。TRP是一种必需氨基酸，其以负担得起的成本广泛可用，从而与单独使用常规培养基相比允许hPSC的成本有效的增殖。在这里，我们描述了用于增强来自真皮成纤维细胞或外周血细胞的hPSC增殖的步骤，但是该方案可以应用于任何hPSC。
We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to. Preparation for TRP-fortified medium from conventional hPSC maintenance medium Replacement of conventional hPSC maintenance medium by TRP-fortified medium Evaluation for proliferation and pluripotency of hPSCs cultured in TRP-fortified medium We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs.
影响因子：
1.6
作者：
Igarashi, Naoko;Onoue, Satomi;Tsuda, Yoshiko
通讯作者：
Tsuda, Yoshiko
影响因子：
5.8
作者：
Someya S;Tohyama S;Kameda K;Tanosaki S;Morita Y;Sasaki K;Kang MI;Kishino Y;Okada M;Tani H;Soma Y;Nakajima K;Umei T;Sekine O;Moriwaki T;Kanazawa H;Kobayashi E;Fujita J;Fukuda K
通讯作者：
Fukuda K