Determination of the refractive index increment (dn/dc) of molecule and macromolecule solutions by surface plasmon resonance
Determination of the refractive index increment (dn/dc) of molecule and macromolecule solutions by surface plasmon resonance
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DOI：
10.1016/j.ab.2004.06.010
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发表时间：
2004-10-15
影响因子：
2.9
通讯作者：
Baptista, MS
中科院分区：
文献类型：
--
作者：
Tumolo, T;Angnes, L;Baptista, MS
提出了一种利用表面等离子体共振仪与流动注射梯度系统（图- spr）串联自动测定dn/dc的方法。小分子和生物分子、表面活性剂、聚合物和生物聚合物溶液的Dn /dc测定精度在1-2%左右，准确度较高。在传统的SPR设备上进行了dn/dc测量，具有相似的精度和精度。FIG-SPR仪器价格低廉，可以很容易地与市售的SPR和液相色谱仪器相结合，以获得基于折射率测量的溶液的几种特性。(C) 2004爱思唯尔公司版权所有。
An automated method for dn/dc determination using a surface plasmon resonance instrument in tandem with a flow injection gradient system (FIG-SPR) is proposed. dn/dc determinations of small molecule and biomolecule, surfactant, polymer, and biopolymer solutions with precision around 1-2% and good accuracy were performed using the new method. dn/dc measurements were also carried out manually on a conventional SPR equipment with similar accuracy and precision. The FIG-SPR instrument is inexpensive and could be easily coupled to commercially available SPR and liquid chromatography instruments to obtain several properties of the solutions, which are based on measurements of refractive index. (C) 2004 Elsevier Inc. All rights reserved.