A shared gene drives lateral root development and root nodule symbiosis pathways in Lotus
A shared gene drives lateral root development and root nodule symbiosis pathways in Lotus
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DOI：
10.1126/science.aax2153
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发表时间：
2019-11-22
期刊：
影响因子：
56.9
通讯作者：
Hayashi, Makoto
中科院分区：
文献类型：
--
作者：
Soyano, Takashi;Shimoda, Yoshikazu;Hayashi, Makoto
豆科植物与固氮根瘤菌共生，形成根瘤。根瘤菌诱导分化的皮质细胞分裂为根瘤原基，以容纳细菌共生体。在这项研究中，我们发现，在日本莲子结瘤过程中，结节起始（NIN）是启动皮质细胞分裂所必需的转录因子，调控基因不对称叶2-LIKE 18/侧器官边界域16a （ASL18/LBD16a）。非豆科植物的侧根发育需要ASL18/LBD16a同源基因。ASL18a和CCAAT盒结合蛋白核因子- y （NF-Y）亚基的共表达，也被NIN直接靶向，部分抑制了日本枇杷突变体的结瘤缺陷表型，其中NIN的皮质表达减弱。我们的研究结果表明ASL18a和NF-Y共同调节结节器官发生。因此，在NIN下游存在一条侧根发育途径来驱动根瘤共生。
Legumes develop root nodules in symbiosis with nitrogen-fixing rhizobial bacteria. Rhizobia evoke cell division of differentiated cortical cells into root nodule primordia for accommodating bacterial symbionts. In this study, we show that NODULE INCEPTION (NIN), a transcription factor in Lotus japonicus that is essential for initiating cortical cell divisions during nodulation, regulates the gene ASYMMETRIC LEAVES 2-LIKE 18/LATERAL ORGAN BOUNDARIES DOMAIN 16a (ASL18/LBD16a). Orthologs of ASL18/LBD16a in nonlegume plants are required for lateral root development. Coexpression of ASL18a and the CCAAT box-binding protein Nuclear Factor-Y (NF-Y) subunits, which are also directly targeted by NIN, partially suppressed the nodulation-defective phenotype of L. japonicus daphne mutants, in which cortical expression of NIN was attenuated. Our results demonstrate that ASL18a and NF-Y together regulate nodule organogenesis. Thus, a lateral root developmental pathway is incorporated downstream of NIN to drive nodule symbiosis.