Functional long circulating single walled carbon nanotubes for fluorescent/photoacoustic imaging-guided enhanced phototherapy
Functional long circulating single walled carbon nanotubes for fluorescent/photoacoustic imaging-guided enhanced phototherapy
复制标题
用于荧光/光声成像引导增强光疗的功能性长循环单壁碳纳米管
DOI：
10.1016/j.biomaterials.2016.06.058
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发表时间：
2016-10-01
期刊：
影响因子：
14
通讯作者：
Zhu, Lei
中科院分区：
文献类型：
--
作者：
Xie, Lisi;Wang, Guohao;Zhu, Lei
纳米治疗已经研究了多年，但临床上只观察到适度的生存益处。这部分归因于静脉内给药后纳米药物的短暂和快速消除。本研究采用新型单壁碳纳米管分散剂伊文思蓝（EB）成功制备了长循环单壁碳纳米管复合物。利用EB与白蛋白之间的高亲和力，自组装负载白蛋白偶联荧光光敏剂二氢卟酚e6（Ce 6），使其具有荧光成像和光动力学治疗能力。所产生的多功能白蛋白/Ce 6负载EB/碳纳米管基递送系统（称为ACEC）能够提供肿瘤的荧光和光声成像以优化治疗时间窗。在注射后24 h，根据成像结果指导进行协同光动力学治疗（PDT）和光热治疗（PTT），并实现有效的肿瘤消融效果。与PDT或PTT单独治疗相比，联合光疗可破坏肿瘤，缩小肿瘤，且无复发。总之，我们的研究提出了一种基于单壁碳纳米管的治疗诊断系统，在双模态成像引导的PTT/PDT联合治疗肿瘤方面具有很大的前景。EB在单壁碳纳米管功能化上的应用可以很容易地扩展到其他纳米材料，以改善其体内稳定性和循环时间。(C)2016爱思唯尔有限公司版权所有。
Nanotherapeutics have been investigated for years, but only modest survival benefits were observed clinic. This is partially attributed to the short and rapid elimination of nanodrug after intravenous administration. In this study, a long circulation single wall carbon nanotube (SWCNT) complex was successfully fabricated through a new SWCNT dispersion agent, evans blue (EB). The complex was endowed with fluorescent imaging and photodynamic therapy ability by self-assembly loading an albumin coupled fluorescent photosensitizer, Chlorin e6 (Ce6) via the high affinity between EB and albumin. The yielding multifunctional albumin/Ce6 loaded EB/carbon nanotube-based delivery system, named ACEC, is capable of providing fluorescent and photoacoustic imaging of tumors for optimizing therapeutic time window. Synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) were carried out as guided by imaging results at 24 h post-injection and achieved an efficient tumor ablation effect. Compared to PDT or PTT alone, the combined phototherapy managed to damage tumor and diminish tumor without recurrence. Overall, our study presents a SWCNT based theranostic system with great promising in dual modalities imaging guided PTT/PDT combined treatment of tumor. The applications of EB on SWCNT functionalization can be easily extended to the other nanomaterials for improving their in vivo stability and circulation time. (C) 2016 Elsevier Ltd. All rights reserved.