Evaluation of reference genes for quantitative real-time PCR in Ostertagia ostertagi by the coefficient of variation and geNorm approach.
Evaluation of reference genes for quantitative real-time PCR in Ostertagia ostertagi by the coefficient of variation and geNorm approach.
复制标题
DOI：
10.1016/j.molbiopara.2007.03.005
复制
发表时间：
2007-06
影响因子：
1.5
通讯作者：
A. V. Zeveren;A. Visser;Prisca R Hoorens;Jozef Vercruysse;E. Claerebout;P. Geldhof
中科院分区：
文献类型：
--
作者：
A. V. Zeveren;A. Visser;Prisca R Hoorens;Jozef Vercruysse;E. Claerebout;P. Geldhof
This paper compares 2 methods, the coefficient of variation and the geNorm software programme, for the evaluation of 5 potential reference genes in the cattle parasiteOstertagia ostertagito be used for quantitative real-time polymerase chain reaction (PCR) analysis. Both methods identifiedTubb(β-tubulin) andGapdh(glyceraldehyde-3-phosphate dehydrogenase) as the most suitable reference genes for the real-time PCR quantification ofO. ostertagi.
