Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection.
Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection.
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DOI：
10.1016/j.ijid.2021.04.018
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Doi Y
中科院分区：
文献类型：
--
作者：
Inaba M;Higashimoto Y;Toyama Y;Horiguchi T;Hibino M;Iwata M;Imaizumi K;Doi Y
逆转录环介导的等温扩增 (RT-LAMP) 已被验证可诊断多种病毒感染。然而，其在现实临床环境中检测 SARS-CoV-2 的诊断准确性仍不清楚。本研究旨在确定 RT-LAMP 与逆转录定量聚合酶链反应 (RT-qPCR) 相比在 COVID-19 病程中的诊断敏感性和特异性。通过 RT-LAMP 和 RT-qPCR 对从 24 名 COVID-19 患者获得的总共 124 份鼻咽拭子样本进行了检测。与 RT-qPCR 相比，RT-LAMP 的敏感性和特异性被分析为发病时间的函数。截至发病后第9天，RT-LAMP的阳性率为92.8%，与RT-qPCR相比，敏感性和特异性均为100%。然而，发病第10天后，RT-LAMP阳性率降至25%以下，两种方法阳性率一致性低于60%。 RT-LAMP 的检测限为 6.7 拷贝/反应。直到症状出现后第 9 天，RT-LAMP 的诊断准确性与 RT-qPCR 相同。这些研究结果表明，RT-LAMP 可用作 COVID-19 的诊断工具，作为 COVID-19 急性症状期 RT-qPCR 的替代方案。
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study aimed to determine the diagnostic sensitivity and specificity of RT-LAMP compared to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) over the disease course of COVID-19. A total of 124 nasopharyngeal swab samples obtained from 24 COVID-19 patients were tested by RT-LAMP and RT-qPCR. Sensitivities and specificities of RT-LAMP compared with RT-qPCR were analyzed as a function of time from onset. Up to the 9th day after onset, the RT-LAMP had a positivity of 92.8%, and the sensitivity and specificity compared with RT-qPCR was 100%. However, after the 10th day after onset, the positivity of RT-LAMP decreased to less than 25%, and the concordance of positivity between the two methods was below 60%. The limit of detection of RT-LAMP was 6.7 copies/reaction. Until the 9th day after the onset of symptoms, RT-LAMP had the same diagnostic accuracy as RT-qPCR. These findings suggest that RT-LAMP can be used as a diagnostic tool for COVID-19 as an alternative to RT-qPCR in the acute symptomatic phase of COVID-19.
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DOI：
10.1073/pnas.2014739117
发表时间：
2020-09-15
影响因子：
11.1
作者：
Ganguli A;Mostafa A;Berger J;Aydin MY;Sun F;Ramirez SAS;Valera E;Cunningham BT;King WP;Bashir R
通讯作者：
Bashir R
开发和验证快速、单步逆转录酶环介导的等温扩增 (RT-LAMP) 系统，有望用于可靠和高通量的 COVID-19 筛查
影响因子：
5.7
作者：
Jiang, Ming-hua;Pan, Wei-hua;Li, X. J.
通讯作者：
Li, X. J.
影响因子：
5.7
作者：
Huang, Wei E.;Lim, Boon;Cui, Zhanfeng
通讯作者：
Cui, Zhanfeng
通过逆转录环介导的等温扩增测定快速直观地检测 2019 新型冠状病毒 (SARS-CoV-2)
影响因子：
14.2
作者：
Yan, C.;Cui, J.;Yuan, J.
通讯作者：
Yuan, J.
在感染期间，什么时候可以检测到SARS-COV-2，并且不再使用基于RT-PCR的测试检测到？对个别参与者数据的系统审查。
影响因子：
9.3
作者：
Mallett S;Allen AJ;Graziadio S;Taylor SA;Sakai NS;Green K;Suklan J;Hyde C;Shinkins B;Zhelev Z;Peters J;Turner PJ;Roberts NW;di Ruffano LF;Wolff R;Whiting P;Winter A;Bhatnagar G;Nicholson BD;Halligan S
通讯作者：
Halligan S