EFFECTS OF Wnt / β-CATENIN SIGNALING PATHWAY AND STAR D7 ON TESTOSTERONE SYNTHESIS.
EFFECTS OF Wnt / β-CATENIN SIGNALING PATHWAY AND STAR D7 ON TESTOSTERONE SYNTHESIS.
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DOI：
10.4183/aeb.2018.155
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
T. Zhang;T. Zheng;C. Wang;W. Zhang;D. Jia;R. Wang;B. Qiao
中科院分区：
文献类型：
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作者：
T. Zhang;T. Zheng;C. Wang;W. Zhang;D. Jia;R. Wang;B. Qiao
【摘要】：背景本研究旨在探讨Wnt/β-catenin信号通路和StarD7促进睾酮合成的机制,探索一条调控睾酮合成的新途径。动物和方法从雄性Sprague-Dawley大鼠中分离Leydig细胞，将其分为四组并用浓度为0、0.1、1和10 nmol/L的膜联蛋白5处理。检测四组大鼠睾酮分泌、StarD7、StarD7 mRNA、β-catenin的表达以及睾丸间质细胞β-catenin定位的变化。结果 用 1 nmol/L 膜联蛋白 5 刺激后，StarD7 和 β-catenin 的 mRNA 和蛋白水平显着增加。在用膜联蛋白 5 处理的细胞中，通过免疫荧光染色观察到 β-catenin 在细胞和细胞核内的积累。这些发现表明 StarD7 和 β-catenin 在膜联蛋白 5 介导的睾酮合成刺激过程中可能发挥作用。结论 Wnt/β-catenin信号通路和StarD7参与了annexin5刺激睾酮合成的过程。 Annexin5 激活 Wnt/β-catenin 信号通路，以及 StarD7 表达增加，导致睾酮合成关键调节酶表达升高，从而促进睾酮合成。
Background This study aimed to assess the mechanism through which Wnt/ beta - catenin signaling pathway, and StarD7, prometes testosterone synthesis, and to explore a new pathway for the regulation of testosterone synthesis. Animals and Methods Leydig cells were isolated from male Sprague-Dawley rats divided into four groups and treated with Annexin 5 in concentration of 0, 0.1, 1 and 10 nmol/L. Testosterone secretion, expression of StarD7, StarD7 mRNA, β-catenin and changes of β - catenin localization in Leydig cells of testis of rats were tested in the four groups. Results mRNA and protein levels of StarD7 and β-catenin increased significantly, upon stimulation with 1 nmol/L annexin 5. Accumulation of β-catenin inside the cells and the nucleus, was observed by immunofluorescence staining, in cells treated with annexin 5. These findings indicate a possible role of StarD7 and β-catenin in the process of annexin5-mediated stimulation of testosterone synthesis. Conclusions Wnt/β-catenin signaling pathway and StarD7 are involved in the process of annexin5 stimulation of testosterone synthesis. Activation of Wnt/ β-catenin signaling pathway by Annexin5, and increase in StarD7 expression lead to elevated expression of key regulatory enzymes in testosterone synthesis, thus promoting testosterone synthesis.