Mosaic analysis of GL2 gene expression and cell layer autonomy during the specification of Arabidopsis leaf trichomes
Mosaic analysis of GL2 gene expression and cell layer autonomy during the specification of Arabidopsis leaf trichomes
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DOI：
10.1002/1526-968x(200010)28:2
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发表时间：
2000-10-01
期刊：
影响因子：
1.5
通讯作者：
Kilby, NJ
中科院分区：
文献类型：
--
作者：
Fyvie, MJ;Murray, JAH;Kilby, NJ
纯合的glabra2（gl2）突变体拟南芥兰兹伯格直立型植株在叶缘仅有少数原始的单尖毛状体，用GL2的基因组克隆对其进行转化后，叶片毛状体的正常表型得到了部分恢复。引入的GL2转基因被构建为一个FLP重组酶响应基因开关的一部分，这使得通过FLP重组酶介导的GL2转基因缺失以及伴随的先前沉默的β -葡萄糖醛酸苷酶（GUS）标记基因的激活，能够产生具有明显标记的gl2突变体克隆区段。贯穿所有三个叶细胞层（L1、L2和L3）的GUS标记区段显示出预期的gl2突变体表型，而紧邻的无标记组织以及覆盖局限于L2和/或L3细胞层的GUS区段的无标记组织则保留了GL2恢复后的表型。这些数据支持GL2基因产物在单个细胞层内以区域自主的方式起作用的观点，并表明L1层中GL2基因的表达足以使毛状体在GL2的作用下向外生长。（C）2000威利 - 利斯公司
Homozygous glabra2 (gl2) mutant Arabidopsis thaliana Landsberg erecta plants with only a few rudimentary single spiked trichomes on the leaf margin were transformed with a genomic clone of GL2, resulting in partial restoration of the normal leaf trichome phenotype. The introduced GL2 transgene was configured as part of an FLP recombinase-responsive gene switch, which permitted visibly marked g12 mutant clonal sectors to be generated by FLP recombinase-mediated deletion of the GL2 transgene with concomitant activation of a previously silent beta -glucuronidase (GUS) marker gene. GUS marked sectors extending through all three leaf cell layers (L1, L2, and L3) displayed the anticipated g12 mutant phenotype, whereas immediately adjacent unmarked tissue, and unmarked tissues overlaying GUS sectors restricted to the L2 and/or L3 cell layers, retained the GL2 restored phenotype, These data support the view that the GL2 gene product acts in a region-autonomous manner within a single cell layer and indicate that GL2 gene expression in the L1 layer is sufficient for GL2-directed outgrowth of trichomes, (C) 2000 Wiley-Liss, Inc.