Ultraviolet and middle wavelength sensitive cone responses in the electroretinogram (ERG) of normal and Rpe65-/- mice
Ultraviolet and middle wavelength sensitive cone responses in the electroretinogram (ERG) of normal and Rpe65-/- mice
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DOI：
10.1016/s0042-6989(01)00140-7
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发表时间：
2001-09-01
期刊：
影响因子：
1.8
通讯作者：
Salchow, DJ
中科院分区：
文献类型：
--
作者：
Ekesten, B;Gouras, P;Salchow, DJ
使用色彩闪光和选择性色彩适应，在正常小鼠和 Rpe65 -/- 小鼠的 ERG 中鉴定出紫外线 (UV) 和中波长敏感 (M) 视锥反应。在正常小鼠中，在亮黄色适应光存在下，紫外线锥反应与在暗淡白光存在下一样大，甚至更大。在黄色适应光存在的情况下，M 锥体反应变得不可检测。黄色适应光最初降低了紫外线响应，但在 8-10 分钟内恢复。 M 锥体反应没有恢复。在 Rpe65 -/- 小鼠中检测不到 UV 锥反应。年轻 Rpe65 -/- 小鼠的 M 锥体反应几乎与正常小鼠一样大。黄色适应光只会减弱这种 M 锥体反应。随着年龄的增长，Rpe -/- 小鼠的 M 锥体反应进一步下降。我们发现 Rpe65 -/- 小鼠中 UV 锥体功能明显丧失，这可能与杆状体共有的 UV 锥体缺陷有关。年轻 Rpe65 -/- 小鼠的 M 锥体功能也已受到影响。黄色适应光对正常小鼠的紫外线锥反应的短暂影响被认为是神经性的，因为它在维持光适应期间消失。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
Ultra-violet (UV) and middle wavelength sensitive (M) cone responses were identified in the ERG of normal and Rpe65 -/- mice using chromatic flashes and selective chromatic adaptation. In normal mice, the UV-cone response was as large as, or larger, in the presence of a bright yellow adapting light than it is in the presence of a dim white light. The M-cone response became undetectable in the presence of the yellow adapting light. Yellow adapting light initially reduced the UV response, but it recovered in 8-10 min. The M-cone response did not recover. UV-cone responses were undetectable in Rpe65 -/- mice. The M-cone response of young Rpe65 -/- mice was almost as large as in normal mice. A yellow adapting light only diminished this M-cone response. With age, the M-cone response further decreased in Rpe -/- mice. We show a pronounced loss of UV-cone function in Rpe65 -/- mice, which may be related to a defect UV-cones share with rods. The M-cone function is also affected already in young Rpe65 -/- mice. The transient effect of a yellow adapting light on the UV-cone response of normal mice is suggested to be neural, because it disappears during maintained light adaptation. (C) 2001 Elsevier Science Ltd. All rights reserved.