Procedure for radiolabeling gizzerosine and basis for a radioimmunoassay.
Procedure for radiolabeling gizzerosine and basis for a radioimmunoassay.
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DOI：
10.1021/jf981336j
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发表时间：
1999-09
影响因子：
6.1
通讯作者：
M. Torres;H. Manosalva;I. Carrasco;A. D. De Ioannes;M. Becker
中科院分区：
文献类型：
--
作者：
M. Torres;H. Manosalva;I. Carrasco;A. D. De Ioannes;M. Becker
介绍了一种标记鱼粉中生物胺吉泽罗辛(GZ)的方法。用水溶性Bolton-Hunter试剂和温和的水不溶氧化剂(Iodogen)试剂标记~(125)I是一种快速和重复性好的方法。用组胺-琼脂糖柱吸附抗GZ多克隆抗体建立的放射免疫分析表明，(125)I-GZ半抗原具有免疫活性。线性范围为0.0001.2~0.1微克/毫升。先前用甲醇提取组胺并进行酸解的鱼粉样品被已知数量的GZ污染，并提交化验。被GZ污染的鱼粉样品表现出与标准曲线相似的剂量-反应效应，显然样品中存在的其他组分不干扰抗体与(125)I-GZ的结合。这些数据表明放射免疫分析法适合于具体测定鱼粉中的GZ。
A method for the labeling of gizzerosine (GZ), a biogenic amine found in fish meal, is described. The labeling procedure with (125)I using a water-soluble Bolton-Hunter reagent and a mild water-insoluble oxidant (Iodogen) reagent is rapid and reproducible. The (125)I-GZ hapten was demonstrated to be immunologically active in a radioimmunoassay developed with polyclonal antibodies to GZ absorbed with a histamine-Sepharose column. The curves were linear in the range of 0.0001 and 0.1 microgram/mL. Samples of fish meal previously extracted of histamine with methanol and submitted to acid hydrolysis were contaminated with known amounts of GZ and submitted to the assay. The fish meal samples contaminated with GZ show a dose-response effect similar to the standard curve, and apparently the other component present in the sample did not interfere with the binding of the antibodies to (125)I-GZ. These data indicate the suitability of the radioimmunoassay to determine specifically GZ in fish meal.