Molecular evidence that the genes for dioecism and monoecism in Spinacia oleracea L. are located at different loci in a chromosomal region
Molecular evidence that the genes for dioecism and monoecism in Spinacia oleracea L. are located at different loci in a chromosomal region
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DOI：
10.1038/hdy.2013.112
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发表时间：
2013-10
期刊：
影响因子：
3.8
通讯作者：
Ken Yamamoto;Y. Oda;A. Haseda;S. Fujito;T. Mikami;Y. Onodera
中科院分区：
文献类型：
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作者：
Ken Yamamoto;Y. Oda;A. Haseda;S. Fujito;T. Mikami;Y. Onodera
菠菜（Spinacia oleracea L.）众所周知是雌雄异株的然而，雌雄同株的植物也可以出现在这个物种中。雌雄异株菠菜的性别表达是由一对基因X和Y控制的。我们以前的研究表明，控制菠菜品系03-336雌雄同株的一对不完全显性基因应该是等位基因或与X/Y连锁。在此，我们开发了19个与雌雄同株基因紧密连锁的AFLP标记。AFLP标记被定位到38.2-cM的染色体区域，该区域包括雌雄同株基因，该基因位于侧翼标记之间，距离为7.1 cM。在我们的研究中开发的四个AFLP标记被转换成序列特征扩增区（SCAR）标记，这是连锁的雌雄同株基因和Y，是共同的两个群体分离的基因。利用SCAR标记进行的连锁分析表明，雌雄同株基因（M）和Y位于不同的标记对之间的不同间隔。对M和Y分离的群体的分析也直接表明了基因在距离为1212 c M处的连锁。本研究的结果可为选育雌雄异株和高度雄性的雌雄同株系作为杂交种子生产的花粉亲本，以及研究该物种有性系统的进化历史提供有用的资料，并可为性别决定基因的定位克隆提供分子基础。
Spinach (Spinacia oleracea L.) is widely known to be dioecious. However, monoecious plants can also occur in this species. Sex expression in dioecious spinach plants is controlled by a single gene pair termed X and Y. Our previous study showed that a single, incompletely dominant gene, which controls the monoecious condition in spinach line 03–336, should be allelic or linked to X/Y. Here, we developed 19 AFLP markers closely linked to the monoecious gene. The AFLP markers were mapped to a 38.2-c M chromosomal region that included the monoecious gene, which is bracketed between flanking markers with a distance of 7.1 c M. The four AFLP markers developed in our studies were converted into sequence-characterized amplified region (SCAR) markers, which are linked to both the monoecious gene and Y and are common to both populations segregating for the genes. Linkage analysis using the SCAR markers suggested that the monoecious gene (M) and Y are located in different intervals, between different marker pairs. Analysis of populations segregating for both M and Y also directly demonstrates linkage of the genes at a distance of∼ 12 c M. The data presented in this study may be useful for breeding dioecious and highly male monoecious lines utilized as the pollen parents for hybrid seed production, as well as for studies of the evolutionary history of sexual systems in this species, and can provide a molecular basis for positional cloning of the sex-determining genes.