Label-retaining cells are preferentially located in fornical epithelium: implications on conjunctival epithelial homeostasis.
Label-retaining cells are preferentially located in fornical epithelium: implications on conjunctival epithelial homeostasis.
复制标题
DOI：
--
复制
发表时间：
1995
影响因子：
4.4
通讯作者：
Zhiqian Wei;G. Cotsarelis;T. Sun;R. M. Lavker
中科院分区：
文献类型：
--
作者：
Zhiqian Wei;G. Cotsarelis;T. Sun;R. M. Lavker
目的测定结膜不同区域上皮细胞的细胞动力学特性。方法对新生和成年SENCAR小鼠的球结膜、穹窿结膜和睑结膜上皮进行形态学和细胞动力学研究。为了检测结膜上皮的增殖率，使用单次给予氚化胸苷（3 H-TdR）来检测处于“S”期的细胞。腹腔注射秋水仙素以阻止细胞有丝分裂后，通过测定有丝分裂活性来评估凋亡率。为了检测慢循环细胞，小鼠连续接受1周的3 H-TdR。在4周追踪后，处死动物并手术切除眼睛。将所有组织立即固定在福尔马林中，并进行组织学和放射自显影处理。结果在眼球、穹窿、眼睑上皮和角膜缘上皮中发现慢周期细胞，即标记保留细胞（LRC）。穹窿上皮中LRC的数量最多。此外，我们还发现了一些标记保留杯状细胞。该细胞群显示在单次脉冲给药后掺入3 H-TdR，并且在秋水仙碱处理后在杯状细胞中观察到有丝分裂像，表明结膜杯状细胞具有增殖能力。结论：这些发现与早期的体外实验数据一致，穹窿上皮可能是一个富含结膜上皮干细胞的区域。这在结膜上皮发育中具有重要意义，并且与伤口修复相关。此外，杯状细胞是具有增殖能力的慢循环细胞的概念为结膜稳态领域提供了新的见解。
PURPOSE To determine the cell kinetic properties of epithelial cells from various zones of the conjunctiva. METHODS The morphology and cell kinetics of bulbar, fornical, and palpebral conjunctival epithelium were studied in neonatal and adult SENCAR mice. To examine the proliferative rate of the conjunctival epithelium, a single administration of tritiated thymidine (3H-TdR) was used to detect cells in "S" phase. Proliferative rates were also assessed by determining mitotic activity after an intraperitoneal injection of colchicine to arrest cells in mitosis. To detect slow-cycling cells, mice received 3H-TdR continuously for 1 week. After a 4-week chase, animals were sacrificed and eyes were surgically removed. All tissues were immediately fixed in formalin and processed for histology and autoradiography. RESULTS Slow-cycling cells, detected as label-retaining cells (LRCs), were identified in bulbar, fornical, and palpebral epithelia, as well as in limbal epithelium. The greatest number of LRCs was found in fornical epithelium. In addition, we found a number of label-retaining goblet cells. This cell population was shown to incorporate 3H-TdR after a single pulse administration, and mitotic figures were seen in goblet cells after colchicine treatment, indicating that conjunctival goblet cells have proliferative capabilities. CONCLUSIONS These findings are consistent with earlier in vitro data that the fornical epithelium may be a zone enriched in conjunctival epithelial stem cells. This has important implications in conjunctival epithelial development and is relevant in wound repair. Furthermore, the concept that goblet cells are slow-cycling cells with proliferative capabilities provides new insights into the area of conjunctival homeostasis.