Antimicrobial susceptibility pattern of Helicobacter suis isolates from pigs and macaques
Antimicrobial susceptibility pattern of Helicobacter suis isolates from pigs and macaques
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DOI：
10.1016/j.vetmic.2019.108459
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发表时间：
2019-12-01
影响因子：
3.3
通讯作者：
De Witte, C.
中科院分区：
文献类型：
--
作者：
Berlamont, H.;Smet, A.;De Witte, C.
猪螺杆菌是一种挑剔的革兰氏阴性细菌，在猪和非人类灵长类动物的胃中定居。用琼脂和肉汤联合稀释法分析了15种抗菌剂对猪和猕猴分离的20株和15株猪链球菌的抗菌活性。微氧培养48h后，用实时定量PCR软件计算细菌生长的最低抑菌浓度(MIC)，β-内酰胺类抗生素、大环内酯类、庆大霉素、新霉素、多西环素、甲硝唑和利福平的MIC呈单峰分布。MIC呈双峰分布，表明2株猪分离株不属于氟喹诺酮类药物野生型种群。四环素的1个猪分离株，林可霉素的1个猪分离株和2个灵长类分离株，以及壮观霉素的1个灵长类分离株也是如此。在不属于氟喹诺酮类药物WTP的菌株中，gyrA基因存在单核苷酸多态(SNPs)；在不属于四环素和壮观霉素WTP的菌株中，存在核糖体蛋白编码基因的单核苷酸多态(SNP)。猪链球菌对氨苄西林、四环素和多西环素的最低抑菌浓度高于灵长类分离株，并在编码青霉素结合蛋白和核糖体蛋白的基因中检测到SNPs。本研究表明，获得性耐药偶尔发生在猪链球菌分离株中，在动物生理学上重要的猪分离株可能比灵长类分离株对β-内酰胺类抗生素和四环素的敏感性更低。
Helicobacter suis is a fastidious, Gram negative bacterium that colonizes the stomach of pigs and non-human primates. It has also been associated with gastric disease in humans.A combined agar and broth dilution method was used to analyze the activity of 15 antimicrobial agents against 20 and 15 H. suis isolates obtained from pigs and macaques, respectively. After 48 h microaerobic incubation, minimal inhibitory concentrations (MICs) were determined by software-assisted calculation of bacterial growth as determined by quantitative real-time PCR.A monomodal distribution of MICs was seen for beta-lactam antibiotics, macrolides, gentamicin, neomycin, doxycycline, metronidazole, and rifampicin. Presence of a bimodal distribution of MICs indicated that 2 porcine isolates did not belong to the wild type population (WTP) for fluoroquinolones. This was also the case for 1 porcine isolate for tetracycline, 1 porcine and 2 primate isolates for lincomycin, and 1 primate isolate for spectinomycin. Single nucleotide polymorphisms (SNPs) were present in the gyrA gene of the isolates not belonging to the WTP for fluoroquinolones and in ribosomal protein encoding genes of the isolates not belonging to the WTP for tetracycline and spectinomycin. MICs of ampicillin, tetracycline and doxycycline were higher for porcine H. suis isolates compared to primate isolates and in these porcine isolates SNPs were detected in genes encoding penicillin binding and ribosomal proteins.This study indicates that acquired resistance occasionally occurs in H. suis isolates and that zoonotically important porcine isolates may be intrinsically less susceptible to beta-lactam antibiotics and tetracyclines than primate isolates.