STRUCTURAL AND BIOCHEMICAL ASPECTS OF CELL MOTILITY IN AMEBAS OF DICTYOSTELIUM-DISCOIDEUM
STRUCTURAL AND BIOCHEMICAL ASPECTS OF CELL MOTILITY IN AMEBAS OF DICTYOSTELIUM-DISCOIDEUM
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DOI：
10.1083/jcb.72.2.339
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发表时间：
1977-01-01
影响因子：
7.8
通讯作者：
RUBIN, RW
中科院分区：
文献类型：
--
作者：
ECKERT, BS;WARREN, RH;RUBIN, RW
Amoeaestra of D.盘状突含有微丝和微管。当甘油化的细胞响应Mg-ATP而收缩时，在皮质纤维网中发现的微丝聚集成束。这些皮层纤维结合重的解肌球蛋白。聚集前的阿米巴中微管稀疏。秋水仙素、灰黄霉素或冷处理不影响细胞运动或细胞形状。这些处理抑制了细胞质颗粒的跳跃运动，并且颗粒积聚在细胞的后部。细胞运动率随阿米巴网囊藻生命周期的不同阶段而变化。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳定量细胞肌动蛋白表明，细胞肌动蛋白的数量在生命周期中发生变化。肌动蛋白的这些变化与运动率的变化直接相关。在饲养阶段结束时向网骨藻培养物中添加环AMP可防止在预聚集阶段期间运动率下降。环AMP也修改在预聚集过程中细胞的肌动蛋白含量的变化。
Amoebae of D. discoideum contain both microfilaments and microtubules. Microfilaments, found in a cortical filament network, aggregate into bundles when glycerinated cells contract in response to Mg-ATP. These cortical filaments bind heavy meromyosin. Microtubules are sparse in amebae before aggregation. Colchicine, griseofulvin, or cold treatments do not affect cell motility or cell shape. Saltatory movement of cytoplasmic particles is inhibited by these treatments and the particles accumulate in the posterior of the cell. Cell motility rate changes as Dictyostelium amebas go through different stages of the life cycle. Quantitation of cellular actin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that the quantity of cellular actin changes during the life cycle. These changes in actin are directly correlated with changes in motility rate. Addition of cyclic AMP to Dictyostelium cultures at the end of the feeding stage prevents a decline in motility rate during the preaggregation stage. Cyclic AMP also modifies the change in actin content of the cells during preaggregation.