TRPC1, A HUMAN HOMOLOG OF A DROSOPHILA STORE-OPERATED CHANNEL
TRPC1, A HUMAN HOMOLOG OF A DROSOPHILA STORE-OPERATED CHANNEL
复制标题
DOI：
10.1073/pnas.92.21.9652
复制
发表时间：
1995-10-10
影响因子：
11.1
通讯作者：
MONTELL, C
中科院分区：
文献类型：
--
作者：
WES, PD;CHEVESICH, J;MONTELL, C
在许多脊椎动物和无脊椎动物细胞中，肌醇1，4，5-三磷酸的产生诱导双相Ca 2+信号。Ca 2+从内部存储的动员驱动初始爆发。第二阶段，称为钙库操作的Ca 2+进入（以前的电容性Ca 2+进入），当细胞内Ca 2+池耗尽激活非电压敏感的质膜Ca 2+电导时发生。尽管钙池操作的钙离子进入的流行，没有脊椎动物通道负责钙池操作的钙离子进入已被报道。trp（瞬时受体电位），果蝇基因所需的光转导，编码的唯一已知的候选人，这样的通道在整个胚胎发育。在这份报告中，我们描述了一个人类同源的色氨酸，TRPC 1的分子特征。TRPC 1（瞬时受体电位通道相关蛋白1）是40%相同的果蝇TRP在大多数的蛋白质和缺乏带电残基的S3跨膜区提出所需的电压传感器在许多电压门控离子通道。TRPC 1在胎儿脑和成人心脏、脑、睾丸和卵巢中表达水平最高。证据还提出，TRPC 1代表了一个家庭的原型相关的人类蛋白质。
In many vertebrate and invertebrate cells, inositol 1,4,5-trisphospate production induces a biphasic Ca2+ signal. Mobilization of Ca2+ from internal stores drives the initial burst. The second phase, referred to as store-operated Ca2+ entry (formerly capacitative Ca2+ entry), occurs when depletion of intracellular Ca2+ pools activates a non-voltage-sensitive plasma membrane Ca2+ conductance. Despite the prevalence of store-operated Ca2+ entry, no vertebrate channel responsible for store-operated Ca2+ entry has been reported. trp (transient receptor potential), a Drosophila gene required in phototransduction, encodes the only known candidate for such a channel throughout phylogeny. In this report, we describe the molecular characterization of a human homolog of trp, TRPC1. TRPC1 (transient receptor potential channel-related protein 1) was 40% identical to Drosophila TRP over most of the protein and lacked the charged residues in the S3 transmembrane region proposed to be required for the-voltage sensor in many voltage-gated ion channels. TRPC1 was expressed at the highest levels in the fetal brain and in the adult heart, brain, testis, and ovaries. Evidence is also presented that TRPC1 represents the archetype of a family of related human proteins.