A barley PHD finger transcription factor that confers male sterility by affecting tapetal development
A barley PHD finger transcription factor that confers male sterility by affecting tapetal development
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DOI：
10.1111/pbi.12181
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发表时间：
2014-08-01
影响因子：
13.8
通讯作者：
Wilson, Zoe A.
中科院分区：
文献类型：
--
作者：
Gomez, Jose Fernandez;Wilson, Zoe A.
控制花粉发育在产生杂交作物和选择性育种方面具有重要的商业重要性，但作物雄性不育的特征基因很少见，目前在大麦中还没有这样的例子。然而，从模式物种的知识翻译现在提供了机会，了解和操纵这些过程中的重要经济作物。我们已经使用的信息在拟南芥中的监管网络，以确定和功能特性的大麦PHD转录因子雄性不育1（MS 1），在花药绒毡层中表达，并在花粉发育过程中发挥了关键作用。比较分析拟南芥、水稻和短柄草基因组，以确定MS 1中的保守区域，用于引物设计以扩增大麦MS 1基因;随后使用RACE-PCR产生全长序列。该基因在四分体晚期和小孢子释放早期之间表现出花药特异的绒毡层表达。HvMS 1基因在大麦中的沉默和过表达导致雄性不育。此外，HvMS 1 cDNA，控制的天然拟南芥MS 1启动子，成功地补充了纯合的MS 1拟南芥突变体。这些结果证实了MS 1功能在高等植物中的保守性，特别是在温带谷物中。这提供了第一个大麦雄性不育基因的例子，这为未来控制大麦雄性育性以开发杂交种提供了一个有价值的工具。
Controlling pollen development is of major commercial importance in generating hybrid crops and selective breeding, but characterized genes for male sterility in crops are rare, with no current examples in barley. However, translation of knowledge from model species is now providing opportunities to understand and manipulate such processes in economically important crops. We have used information from regulatory networks in Arabidopsis to identify and functionally characterize a barley PHD transcription factor MALE STERTILITY1 (MS1), which expresses in the anther tapetum and plays a critical role during pollen development. Comparative analysis of Arabidopsis, rice and Brachypodium genomes was used to identify conserved regions in MS1 for primer design to amplify the barley MS1 gene; RACE-PCR was subsequently used to generate the full-length sequence. This gene shows anther-specific tapetal expression, between late tetrad stage and early microspore release. HvMS1 silencing and overexpression in barley resulted in male sterility. Additionally, HvMS1 cDNA, controlled by the native Arabidopsis MS1 promoter, successfully complemented the homozygous ms1 Arabidopsis mutant. These results confirm the conservation of MS1 function in higher plants and in particular in temperate cereals. This has provided the first example of a characterized male sterility gene in barley, which presents a valuable tool for the future control of male fertility in barley for hybrid development.