Cell-Surface Lectins of Azospirillum spp.
Cell-Surface Lectins of Azospirillum spp.
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固氮螺菌属细胞表面凝集素。
作者：
T. Castellanos;F. Ascencio;Y. Bashan
抽象的。从巴西固氮菌和巴西固氮菌中筛选出7株细胞表面凝集素。Lipoferum。凝集素的存在下，通过颗粒凝集试验，采用乳胶珠包被与neoglycoproteins和蛋白质印迹与neoglycoproteins标记辣根过氧化物酶作为探针。7个菌株与测定的糖残基凝集。与岩藻糖和葡萄糖的凝集作用最高，与甘露糖残基的凝集作用程度较小。从两种固氮菌中提取细胞壁蛋白菌株表现出凝集素样活性。我们认为凝集素存在于固氮菌属的细胞壁中。
Abstract. Cell-surface lectins were screened in seven strains of Azospirillum brasilense and A. lipoferum. The presence of lectins was determined by particle agglutination assays employing latex beads coated with neoglycoproteins and by Western blot with neoglycoproteins labeled with horseradish peroxidase as a probe. Seven strains were agglutinated with the assayed sugar residues. The highest agglutination was with fucose and glucose and to a lesser extent with mannose residues. Cell-wall proteins extracted from two Azospirillum spp. strains exhibit lectin-like activities. We believe that lectins are present in the cell-wall of Azospirillum spp.