Role of Autologous Rabbit Adipose-Derived Stem Cells in the Early Phases of the Repairing Process of Critical Bone Defects
Role of Autologous Rabbit Adipose-Derived Stem Cells in the Early Phases of the Repairing Process of Critical Bone Defects
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DOI：
10.1002/jor.21184
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Brini, A. T.
中科院分区：
文献类型：
--
作者：
de Girolamo, L.;Arrigoni, E.;Brini, A. T.
脂肪来源的干细胞(ASCs)可能是促进骨再生的一种新的有效工具。本研究在体外扩增兔ASCs，并将其用于12只新西兰兔胫骨近端骨痂全层骨缺损的修复。移植材料分别为：未处理(对照组)、空白羟基磷灰石(HA)盘、单纯ASCs和植入ASCs的HA盘。每个分离的A、SCs群体都在体外进行了检测：它们都表现出高的增殖率、显著的克隆形成能力和成骨分化潜能。植入8周后，所有样本的大体分析显示，植入细胞组和未植入细胞组之间在硬挺度方面没有明显差异(p>；0.05)，所有样本都得到了满意的填充。在支架处理的两组中，放射学观察到良好的骨结合。即使HA没有被完全吸收，负载ASCs的HA也表现出比未负载的HA更高的支架吸收率。组织学分析表明，支架处理后的缺损区的成骨能力明显高于未支架组，尤其是在ASCs存在的情况下，新生骨更成熟，更接近于自然骨。这些结果表明，自体ASCs HA构建物是一种潜在的骨缺损修复方法。(C)2010年整形外科研究会。由Wiley期刊公司出版，J Orthop Res 29：100-108,2011
Adipose-derived stem cells (ASCs) may represent a novel and efficient tool to promote bone regeneration. In this study, rabbit ASCs were expanded in culture and used for the regeneration of full-thickness bone defects in the proximal epiphysis of tibia of 12 New Zealand rabbits. Defects were implanted with graft material as follows: untreated (control), empty hydroxyapatite (HA) disk, ASCs alone, and HA disk seeded with ASCs. Each isolated A,SCs population was tested in vitro: they all showed a high proliferation rate, a marked clonogenic ability, and osteogenic differentiation potential. Eight weeks after implantation, macroscopic analyses of all the samples showed satisfactory filling of the lesions without any significant differences in term of stiffness between groups treated with or without cells (p > 0.05). In both the scaffold-treated groups, a good osteointegration was radiographically observed. Even if HA was not completely reabsorbed, ASCs-loaded HA displayed a higher scaffold resorption than the unloaded ones. Histological analyses showed that the osteogenic abilities of the scaffold-treated defects was greater than those of scaffold-free samples, and in particular new formed bone was more mature and more similar to native bone in presence of ASCs. These results demonstrated that autologous ASCs HA constructs is a potential treatment for the regeneration of bone defects. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 100-108, 2011