Autoantibody profiles in the sera of European patients with myositis
Autoantibody profiles in the sera of European patients with myositis
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DOI：
10.1136/ard.60.2.116
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发表时间：
2001-02-01
影响因子：
27.4
通讯作者：
van Venrooij, WJ
中科院分区：
文献类型：
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作者：
Brouwer, R;Hengstman, GJD;van Venrooij, WJ
目的：确定肌炎特异性自身抗体（MSAs）和几种肌炎相关自身抗体（MAAs）在一大群肌炎患者中的患病率。（198例多发性肌炎（PM）、181例皮肌炎（DM）和38例包涵体肌炎（IBM）患者）通过免疫印迹进行血清学分析，结果232例（56%）血清中检出自身抗体，其中157例（38%）含有MSA。最常检测到的MSA是抗Jo-1（18%）。其他抗合成酶、抗Mi-2和抗SRP自身抗体分别见于3%、14%和5%的血清中。发现了相对高数量的抗Mi-2阳性PM血清（PM血清的9%）。最常检测到的MAA是抗Ro52（25%）。抗PM/Sc1 - 100、抗PM/Sc1 - 75、抗Mas、抗Ro60、抗La和抗U1 snRNP自身抗体分别占血清的6%、3%、2%、4%、5%和6%。显着的协会之间注意到抗Ro52和抗Jo-1自身抗体，并在少数血清中，抗Jo-1和抗SRP或抗Mi-2自身antibodies.Conclusions之间的发病率大多数测试的自身抗体活动在这一大组欧洲患者是在日本和美国患者的类似研究。具有抗Mi-2反应性的PM血清的相对高的数量可以通过使用跨越完整抗原的多个重组片段来解释。此外，我们的数据表明，一些血清可能含有一种以上的MSA类型，并确认抗Ro52与抗Jo-1反应性的强关联。
Objective-To determine the prevalence of myositis specific autoantibodies (MSAs) and several myositis associated autoantibodies (MAAs) in a large group of patients with myositis.Methods-A total of 417 patients with myositis from 11 European countries (198 patients with polymyositis (PM), 181 with dermatomyositis (DM),and 38 with inclusion body myositis (IBM)) were serologically analysed by immunoblot, enzyme linked immunosorbent assay (ELISA) and/or immunoprecipitation.Results-Autoantibodies were found in 232 sera (56%), including 157 samples (38%) which contained MSAs. The most commonly detected MSA was anti-Jo-1 (18%). Other anti-synthetase, anti-Mi-2, and anti-SRP autoantibodies were found in 3%, 14%, and 5% of the sera, respectively. A relatively high number of anti-Mi-2 positive PM sera were found (9% of PM sera). The most commonly detected MAA was anti-Ro52 (25%). Anti-PM/Sc1-100, anti-PM/Sc1-75, anti-Mas, anti-Ro60, anti-La, and anti-U1 snRNP autoantibodies were present in 6%, 3%, 2%, 4%, 5%, and 6% of the sera, respectively. Remarkable associations were noticed between anti-Ro52 and anti-Jo-1 autoantibodies and, in a few sera, also between anti-Jo-1 and anti-SRP or anti-Mi-2 autoantibodies.Conclusions-The incidence of most of the tested autoantibody activities in this large group of European patients is in agreement with similar studies of Japanese and American patients. The relatively high number of PM sera with anti-Mi-2 reactivity may be explained by the use of multiple recombinant fragments spanning the complete antigen. Furthermore, our data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity.