Identification and Characterization of MicroRNAs from Barley (Hordeum vulgare L.) by High-Throughput Sequencing
Identification and Characterization of MicroRNAs from Barley (Hordeum vulgare L.) by High-Throughput Sequencing
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DOI：
10.3390/ijms13032973
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发表时间：
2012-03-01
影响因子：
5.6
通讯作者：
Weining, Song
中科院分区：
文献类型：
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作者：
Lv, Shuzuo;Nie, Xiaojun;Weining, Song
microRNA（miRNAs）是一类内源性RNA，参与调节多种生物和代谢过程中的基因表达。大麦是世界上最重要的谷类作物之一，是小麦族物种遗传和基因组研究的模式生物。然而，大麦中的miRNA研究相对于禾本科其他模式植物还相对滞后。为了获得大麦中miRNA基因的更多信息，我们使用Solexa测序对来自四种不同组织的等量RNA池创建的小RNA文库进行测序。除了126个保守的miRNAs（58个家族）外，还从该序列数据集中鉴定出133个新的miRNAs，分别属于50个家族。另外还发现了15个新的miRNAs的miRNAs * 序列，为这些miRNAs的存在提供了新的证据。使用qRT-PCR检测6种随机选择的miRNA的表达模式。另外还鉴定了一些参与干旱和盐胁迫反应的miRNAs。此外，使用psRNATarget工具预测这些推定的miRNA的潜在靶点。我们的研究结果显著增加了大麦中新的miRNAs的数量，这将有助于进一步研究大麦和其他物种中miRNAs的生物学功能和进化。
MicroRNAs (miRNAs) are a class of endogenous RNAs that regulates the gene expression involved in various biological and metabolic processes. Barley is one of the most important cereal crops worldwide and is a model organism for genetic and genomic studies in Triticeae species. However, the miRNA research in barley has lagged behind other model species in grass family. To obtain more information of miRNA genes in barley, we sequenced a small RNA library created from a pool of equal amounts of RNA from four different tissues using Solexa sequencing. In addition to 126 conserved miRNAs (58 families), 133 novel miRNAs belonging to 50 families were identified from this sequence data set. The miRNA* sequences of 15 novel miRNAs were also discovered, suggesting the additional evidence for existence of these miRNAs. qRT-PCR was used to examine the expression pattern of six randomly selected miRNAs. Some miRNAs involved in drought and salt stress response were also identified. Furthermore, the potential targets of these putative miRNAs were predicted using the psRNATarget tools. Our results significantly increased the number of novel miRNAs in barley, which should be useful for further investigation into the biological functions and evolution of miRNAs in barley and other species.