Generation of tribenuron‐methyl herbicide‐resistant OsCYP81A6‐expressing rapeseed (Brassica napus L.) plants for hybrid seed production using chemical‐induced male sterility
Generation of tribenuron‐methyl herbicide‐resistant OsCYP81A6‐expressing rapeseed (Brassica napus L.) plants for hybrid seed production using chemical‐induced male sterility
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DOI：
10.1111/pbr.12361
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发表时间：
2016-06
期刊：
影响因子：
2
通讯作者：
Xuan Yao;Yaqin Wang;Xiao-Li Yue;Meng Liu;Kede Liu
中科院分区：
文献类型：
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作者：
Xuan Yao;Yaqin Wang;Xiao-Li Yue;Meng Liu;Kede Liu
化学诱导油菜雄性不育（CIMS）是一种利用化学诱导技术培育甘蓝型油菜雄性不育的方法。生产一些磺酰脲类除草剂如苯磺隆（TBM）被用作CIMS系统中的化学杂交剂（CHA）。但是，在用CHA喷洒母本以诱导雄性不育时，必须用护罩保护父本免受除草剂伤害。因此，利用抗除草剂油菜品系作父本，可以大大简化制种程序，降低制种成本。水稻细胞色素P450羟化酶OsCYP 81 A6以前已被表征为赋予对苯达松和磺酰脲除草剂的抗性。我们在此证明了0 sCYP 81 A6的引入使得油菜植物对TBM具有抗性。与野生型植物相比，当用0.05 mg/l的TBM（用于在杂交种子生产中诱导雄性不育的剂量）处理时，转基因植物显示正常的雄蕊发育和雄性育性。这些结果表明，表达0 sCYP 81 A6的油菜植物可用作利用CIMS生产杂交油菜的父本。
Chemical-induced male sterility (CIMS) is a method for hybrid rapeseed (Brassica napus L.) production. Some sulphonylurea herbicides such as tribenuron-methyl (TBM) are used as chemical hybridization agents (CHAs) in CIMS systems. However, the male parents must be protected from herbicide injury with a shield during spraying of the female parents with CHAs to induce male sterility. Thus, using herbicide-resistant rapeseed lines as the male parents can significantly simplify the seed production procedure and reduce the cost in hybrid seed production. A rice cytochrome P450 hydroxylase, OsCYP81A6, has been previously characterized to confer resistance to bentazon and sulphonylurea herbicides. We demonstrate here that the introduction of OsCYP81A6 renders rapeseed plants resistant to TBM. Compared with wild-type plants, the transgenic plants displayed normal stamen development and male fertility when treated with 0.05 mg/l of TBM, the dose used for inducing male sterility in hybrid seed production. These results indicate that the OsCYP81A6-expressing rapeseed plants can be used as the male parents for hybrid rapeseed production using CIMS.