EFFECT OF SERIAL EMBRYO PASSAGE OF AN ARKANSAS-TYPE AVIAN INFECTIOUS-BRONCHITIS VIRUS ISOLATE ON CLINICAL-RESPONSE, VIRUS RECOVERY, AND IMMUNITY
EFFECT OF SERIAL EMBRYO PASSAGE OF AN ARKANSAS-TYPE AVIAN INFECTIOUS-BRONCHITIS VIRUS ISOLATE ON CLINICAL-RESPONSE, VIRUS RECOVERY, AND IMMUNITY
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DOI：
10.2307/1590310
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发表时间：
1983-01-01
期刊：
影响因子：
1.4
通讯作者：
CLOUD, SS
中科院分区：
文献类型：
--
作者：
GELB, J;CLOUD, SS
传染性支气管炎病毒（IBV）阿肯色型DPI株（Ark DPI）通过鸡胚的连续传代得到了减毒效果。通过对疫苗接种的临床反应、病毒在呼吸道（气管）和非呼吸道（肾脏和排气管）组织中的分离以及体重增加的研究，可以确定50代病毒对日龄母免疫肉鸡型鸡的致病性低于10代或25代病毒。接种10、25或50代病毒的鸡在接种后4和6周对上呼吸道同源病毒的攻击至少有80%的抵抗力。50代疫苗接种的鸡在4周时血清抗体产生相对较低，但在6周时与接种10代或25代病毒的鸡相似。
Infectious bronchitis virus (IBV) Arkansas-type DPI strain (Ark DPI) was attenuated by serial passage in chicken embryos. Virus of passage 50 was less pathogenic for day old maternally immune broiler-type chickens than virus of passage 10 or 25 as determined by clinical response to vaccination, virus isolation in respiratory (trachea) and nonrespiratory (kidney and cloaca) tissues, and weight gain studies. Chickens vaccinated with virus of passage 10, 25 or 50 were at least 80% resistant to homologous virus challenge of the upper respiratory tract 4 and 6 wk postvaccination. Serum antibody production by passage 50-vaccinated chickens was comparatively low at 4 wk but at 6 wk was similar to that in chickens vaccinated with virus of passage 10 or 25.