Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h(-1)) immunomagnetic sorting.
Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h(-1)) immunomagnetic sorting.
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DOI：
10.1039/c6lc00487c
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发表时间：
2016-08-02
期刊：
影响因子：
6.1
通讯作者：
Issadore D
中科院分区：
文献类型：
--
作者：
Ko J;Yelleswarapu V;Singh A;Shah N;Issadore D
微流控设备能够以比传统方法更高的灵敏度和特异度来分选免疫磁性标记的细胞，这主要是因为微流控通道和微尺度磁铁的大小可以与单个细胞的大小相匹配。然而，这些小的特征尺寸是以有限的吞吐量(ϕ<；5毫升h−1)和易堵塞为代价的，这阻碍了当前的微流控技术处理相关数量的临床样本，例如V&gT；10毫升全血。在这里，我们报告了一种新的微磁分选方法，它可以在未经处理的复杂样品中实现高度特定的细胞分离，吞吐量(ϕ>；100mLh−1)比传统微流体高100倍。为了实现这一目标，我们设计了一种新的微磁分选方法-磁性镍铁电铸捕获器(磁体)，它通过使数百万个微磁性捕捉器并行运行来实现高流速。我们的设计将传统的微流体方法旋转90°，在气孔边缘而不是通道内形成磁性陷阱，使数百万个磁性陷阱能够整合到一个厘米大小的设备中。与以前的工作不同，在以前的工作中，磁性结构是通过传统的微细加工来定义的，我们从软光刻中获得了灵感，创造了一种母版，可以经济地制造许多复制的电铸磁性微孔器件。这些独立的12微米厚的坡莫合金(Ni80Fe20)薄膜包含任意形状和位置的微孔，使设备能够定制以实现最大的捕获效率和吞吐量。我们通过制造具有圆形和矩形气孔的设备展示了磁铁的能力，并使用这些设备快速(ϕ=180mLh−1)从白细胞中特异性地分选稀有肿瘤细胞。
Microfluidic devices can sort immunomagnetically labeled cells with sensitivity and specificity much greater than that of conventional methods, primarily because the size of microfluidic channels and micro-scale magnets can be matched to that of individual cells. However, these small feature sizes come at the expense of limited throughput (ϕ < 5 mL h−1) and susceptibility to clogging, which have hindered current microfluidic technology from processing relevant volumes of clinical samples, e.g. V > 10 mL whole blood. Here, we report a new approach to micromagnetic sorting that can achieve highly specific cell separation in unprocessed complex samples at a throughput (ϕ > 100 mL h−1) 100× greater than that of conventional microfluidics. To achieve this goal, we have devised a new approach to micromagnetic sorting, the magnetic nickel iron electroformed trap (MagNET), which enables high flow rates by having millions of micromagnetic traps operate in parallel. Our design rotates the conventional microfluidic approach by 90° to form magnetic traps at the edges of pores instead of in channels, enabling millions of the magnetic traps to be incorporated into a centimeter sized device. Unlike previous work, where magnetic structures were defined using conventional microfabrication, we take inspiration from soft lithography and create a master from which many replica electroformed magnetic micropore devices can be economically manufactured. These free-standing 12 µm thick permalloy (Ni80Fe20) films contain micropores of arbitrary shape and position, allowing the device to be tailored for maximal capture efficiency and throughput. We demonstrate MagNET's capabilities by fabricating devices with both circular and rectangular pores and use these devices to rapidly (ϕ = 180 mL h−1) and specifically sort rare tumor cells from white blood cells.