Shape-dependent gold nanoparticle interactions with a model cell membrane.
Shape-dependent gold nanoparticle interactions with a model cell membrane.
复制标题
DOI：
10.1116/6.0002183
复制
发表时间：
2022-11
期刊：
影响因子：
2.1
通讯作者：
T. W. Golbek;B. Harper;S. Harper;J. Baio
中科院分区：
文献类型：
--
作者：
T. W. Golbek;B. Harper;S. Harper;J. Baio
可定制的金纳米颗粒平台由于其生物兼容性、稳定性和成像能力，正在激励药物发现方面的创新，具有巨大的治疗潜力。进一步的发展需要了解形状、电荷或表面化学的离散差异如何影响纳米颗粒的药物传递过程。纳米颗粒的形状会对纳米颗粒的功能产生重大影响，例如，这会极大地改变可用于修饰的表面积，例如表面配体密度。为了研究纳米粒子形状对细胞膜结构的影响，我们用一种称为和频产生(SFG)振动光谱的界面敏感技术直接探测了纳米粒子与脂质之间的相互作用。金纳米星和带有正电荷配体的金纳米球都被允许与模型细胞膜相互作用，并通过与脂类中分子键相关的特定SFG振动模式直接观察到膜结构的变化。SFG结果表明，+Au纳米星既穿透并影响了构成膜的脂类的有序性，而+Au纳米球与模型膜相互作用时，模型膜的结构变化很小。这表明，与+Au纳米球相比，+Au纳米星对细胞膜的破坏更大。我们的发现表明了形状在纳米材料设计中的重要性，并提供了强有力的证据，表明形状在定义纳米材料与生物的相互作用中确实发挥了作用。
Customizable gold nanoparticle platforms are motivating innovations in drug discovery with massive therapeutic potential due to their biocompatibility, stability, and imaging capabilities. Further development requires the understanding of how discrete differences in shape, charge, or surface chemistry affect the drug delivery process of the nanoparticle. The nanoparticle shape can have a significant impact on nanoparticle function as this can, for example, drastically change the surface area available for modifications, such as surface ligand density. In order to investigate the effects of nanoparticle shape on the structure of cell membranes, we directly probed nanoparticle-lipid interactions with an interface sensitive technique termed sum frequency generation (SFG) vibrational spectroscopy. Both gold nanostars and gold nanospheres with positively charged ligands were allowed to interact with a model cell membrane and changes in the membrane structure were directly observed by specific SFG vibrational modes related to molecular bonds within the lipids. The SFG results demonstrate that the +Au nanostars both penetrated and impacted the ordering of the lipids that made up the membrane, while very little structural changes to the model membrane were observed by SFG for the +Au nanospheres interacting with the model membrane. This suggests that the +Au nanostars, compared to the +Au nanospheres, are more disruptive to a cell membrane. Our findings indicate the importance of shape in nanomaterial design and provide strong evidence that shape does play a role in defining nanomaterial-biological interactions.