Myb-dependent Regulation of Thrombospondin 2 Expression
Myb-dependent Regulation of Thrombospondin 2 Expression
复制标题
血小板反应蛋白 2 表达的 Myb 依赖性调节
DOI：
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发表时间：
1998
影响因子：
4.8
通讯作者：
M. Simons
中科院分区：
文献类型：
--
作者：
Kiflai Bein;J. Ware;M. Simons
The nuclear transcription factor c-Myb, which is highly expressed in hematopoietic cells, has been shown to be functional in NIH 3T3 cells: cells that do not possess detectable levels of c-Myb. To identify endogenous target genes of c-Myb in fibroblasts, RNA isolated from NIH 3T3 cells stably transfected with a full-length or a dominant negative c-myb construct (GREMyb and GREMEn, respectively) was subjected to differential display analysis. 5′-Rapid amplification of cDNA ends of a selected band, sequencing, and a nucleotide homology search led to the identification of thrombospondin 2 (TSP 2) as the gene product repressed in GREMyb and induced in GREMEn cells. The pattern of TSP 2 expression during the cell cycle was consistent with c-myb-dependent regulation. The possibility that the identified transcript was TSP 1, a homologous product known to be repressed by v-Src, c-Jun, and v-Myc, was ruled out by using a TSP 2-specific DNA probe and by showing a distinct pattern of regulation of TSP 1 and TSP 2 expression. Nuclear run-on and TSP 2 promoter-reporter (chloramphenicol acetyltransferase) assays showed similar transcriptional levels in GREMyb and NIH 3T3 cells. However, mRNA stability studies showed a much shorter TSP 2 mRNA half-life in GREMyb compared with wild type NIH 3T3 cells, suggesting that c-myb affects TSP 2 expression via a post-transcriptional mechanism. The implications of a protooncogene-mediated suppression of TSP expression are discussed.
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DOI：
10.1152/ajpcell.1995.268.4.c856
发表时间：
1995-04
期刊：
The American journal of physiology
影响因子：
--
作者：
M. Simons;H. Ariyoshi;E. Salzman;R. Rosenberg
通讯作者：
M. Simons;H. Ariyoshi;E. Salzman;R. Rosenberg
在斜纹夜蛾细胞中表达的重组小鼠血小板反应蛋白 2 的特性。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen,H;Sottile,J;O'Rourke,KM;Dixit,VM;Mosher,DF
通讯作者：
Mosher,DF
c-myb mRNA 表达的组成型与细胞周期调节与小鼠 B 淋巴肿瘤的发育阶段相关。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Catron,KM;Purkerson,JM;Isakson,PC;Bender,TP
通讯作者：
Bender,TP
DOI：
10.1073/pnas.87.17.6624
发表时间：
1990-09-01
影响因子：
11.1
作者：
GOOD, DJ;POLVERINI, PJ;BOUCK, NP
通讯作者：
BOUCK, NP
DOI：
10.1152/ajpcell.1997.272.6.c1947
发表时间：
1997-06-01
影响因子：
5.5
作者：
Husain, M;Jiang, LW;Rosenberg, RD
通讯作者：
Rosenberg, RD