WAVE-DAMPENED2-LIKE4 modulates the hyper-elongation of light-grown hypocotyl cells
WAVE-DAMPENED2-LIKE4 modulates the hyper-elongation of light-grown hypocotyl cells
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DOI：
10.1093/plphys/kiad248
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发表时间：
2023-04-25
期刊：
影响因子：
7.4
通讯作者：
Shaw, Sidney L.
中科院分区：
文献类型：
--
作者：
Schaefer, Kristina;Baza, Ariadna Cairo;Shaw, Sidney L.
微管相关蛋白限制了下胚轴细胞在光照和高温下的生长，并调节激素敏感性，而不会对微管阵列图案产生重大影响。光、温度、水和养分的可用性影响植物如何生长以最大限度地获得资源。轴向生长，协调轴向细胞扩张的组织的线性延伸，在这些适应性形态反应中起着核心作用。使用拟南芥（拟南芥）下胚轴细胞探索轴向生长控制机制，我们研究了WAVE-DAMPENED 2-LIKE 4（WDL 4），生长素诱导的微管相关蛋白和更大的WDL基因家族的成员，在不断变化的环境条件下调节下胚轴生长。功能丧失的wdl 4幼苗在光照条件下表现出超伸长表型，当野生型Col-0下胚轴停滞时继续伸长，并在芽出现之前达到野生型长度的150%至200%。WDL 4幼苗下胚轴响应温度升高显示出显著的超伸长（500%），表明在形态学对环境线索的适应中起重要作用。WDL 4在光照和黑暗生长条件下都与微管相关，并且没有发现在各种条件下功能丧失的WDL 4突变体中改变微管阵列图案的证据。激素反应的检查显示改变的敏感性乙烯和生长素依赖的转录报告的空间分布的变化的证据。我们的数据提供的证据表明，WDL 4调节下胚轴细胞伸长没有实质性的变化，微管阵列图案，这表明在轴向生长控制的非常规作用。
A microtubule associated protein limits hypocotyl cell growth in the light and at elevated temperatures and regulates hormone sensitivity without major changes to microtubule array patterning.Light, temperature, water, and nutrient availability influence how plants grow to maximize access to resources. Axial growth, the linear extension of tissues by coordinated axial cell expansion, plays a central role in these adaptive morphological responses. Using Arabidopsis (Arabidopsis thaliana) hypocotyl cells to explore axial growth control mechanisms, we investigated WAVE-DAMPENED2-LIKE4 (WDL4), an auxin-induced, microtubule-associated protein and member of the larger WDL gene family shown to modulate hypocotyl growth under changing environmental conditions. Loss-of-function wdl4 seedlings exhibited a hyper-elongation phenotype under light conditions, continuing to elongate when wild-type Col-0 hypocotyls arrested and reaching 150% to 200% of wild-type length before shoot emergence. wdl4 seedling hypocotyls showed dramatic hyper-elongation (500%) in response to temperature elevation, indicating an important role in morphological adaptation to environmental cues. WDL4 was associated with microtubules under both light and dark growth conditions, and no evidence was found for altered microtubule array patterning in loss-of-function wdl4 mutants under various conditions. Examination of hormone responses showed altered sensitivity to ethylene and evidence for changes in the spatial distribution of an auxin-dependent transcriptional reporter. Our data provide evidence that WDL4 regulates hypocotyl cell elongation without substantial changes to microtubule array patterning, suggesting an unconventional role in axial growth control.