PCR and oligonucleotide array for detection of Enterobacter sakazakii in infant formula
PCR and oligonucleotide array for detection of Enterobacter sakazakii in infant formula
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DOI：
10.1016/j.mcp.2005.08.004
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Huang, XT
中科院分区：
文献类型：
--
作者：
Liu, Y;Gao, QL;Huang, XT
坂崎肠杆菌与几种新生儿脑膜炎有关，死亡率很高。本研究建立了一种特异性PCR和寡核苷酸芯片技术检测大肠杆菌16 S-23 SrDNA内转录间隔区（ITS）的方法。坂崎。设计了2对特异性PCR引物和10条寡核苷酸探针，对6株大肠杆菌的ITS序列进行了测定。sakazakii和GenBank的BLAST。针对来自88种不同细菌菌株的一组众多菌株测试PCR和寡核苷酸阵列方法的特异性和效率。所有的E。坂崎株与非E.利用PCR和寡核苷酸芯片技术对坂崎菌株进行ITS序列检测。检测灵敏度为1.3 CFU/100 g婴儿配方奶粉。PCR和寡核苷酸阵列程序（包括富集培养）仅需48小时，而传统方法至少需要5天。本研究表明，这两种病原体检测是省时和可靠的。(c)2005爱思唯尔有限公司保留所有权利。
Enterobacter sakazakii has been implicated in a several form of neonatal meningitis with a high mortality rate. In the present study, the species-specific PCR and oligonucleotide array assays were developed to detect the 16S-23S rDNA internal transcribed spacer (ITS) of E. sakazakii. Two pairs of specific PCR primers and 10 oligonucleotide probes were designed by sequencing the ITS of six strains of E. sakazakii and BLAST of GenBank. The specificity and efficiency of the PCR and oligonucleotide array methods were tested against a panel of numerous strains from 88 different bacterial strains. All of the E. sakazakii strains generated positive signal, and no cross-reaction was observed with non-E. sakazakii strains in the PCR and oligonLiCleotide array detections based on ITS sequences. Sensitivity of the detections is 1.3 CFU/100 g infant formula with the selective enrichment. Both of the PCR and oligonucleotide array procedures take only 48 h including the enrichment culture, whereas the conventional methods required at least 5 days. This study demonstrated that both of the pathogenic detections are time-saved and reliable. (c) 2005 Elsevier Ltd. All rights reserved.