Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions
Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions
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DOI：
10.1093/jxb/erz462
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发表时间：
2020-01-07
影响因子：
6.9
通讯作者：
Singla-Pareek, Sneh Lata
中科院分区：
文献类型：
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作者：
Joshi, Rohit;Sahoo, Khirod Kumar;Singla-Pareek, Sneh Lata
土壤因素，如盐、碱和干旱，单独或组合对作物生产力产生不利影响。尽管土壤盐碱化被报道为全球范围内日益严重的问题，但为解决这一问题所做的努力有限。在本研究中，我们的目的是产生耐盐碱水稻与耐盐和干旱。利用E.在ABA诱导型启动子的控制下，我们将编码海藻糖-6-磷酸合酶/磷酸酶（TPSP）的大肠杆菌转化为无标记的高产转基因水稻（在IR 64背景下），该转基因水稻能够耐受高pH（类似于9.9）、高EC（类似于10.0 dS m（-1））和严重干旱（30-35%土壤水分含量）。转基因植株保持了较高的相对含水量（RWC）、叶绿素含量、K+/Na* 比值、气孔数目和气孔长度。电导和光合效率相比，野生型在这些压力。在干旱、盐碱和苏打条件下，海藻糖过量生产和高产参数之间呈正相关。使用GC-MS的代谢分析表明，过量生产的海藻糖在叶片中不同地调制其他代谢开关，导致糖，氨基酸和有机酸的水平在控制和胁迫条件下的转基因植物中的显着变化。我们的研究结果揭示了一种新的潜在技术解决方案，可以在不断变化的气候条件下应对多重压力。
Edaphic factors such as salinity, sodicity, and drought adversely affect crop productivity, either alone or in combination. Despite soil sodicity being reported as an increasing problem worldwide, limited efforts have been made to address this issue. In the present study, we aimed to generate rice with tolerance to sodicity in conjunction with tolerance to salinity and drought. Using a fusion gene from E. coli coding for trehalose-6-phosphate synthase/phosphatase (TPSP) under the control of an ABA-inducible promoter, we generated marker-free, high-yielding transgenic rice (in the IR64 background) that can tolerate high pH (similar to 9.9), high EC (similar to 10.0 dS m(-1)), and severe drought (30-35% soil moisture content). The transgenic plants retained higher relative water content (RWC), chlorophyll content, K+/Na* ratio, stomata! conductance, and photosynthetic efficiency compared to the wild-type under these stresses. Positive correlations between trehalose overproduction and high-yield parameters were observed under drought, saline, and sodic conditions. Metabolic profiling using GC-MS indicated that overproduction of trehalose in leaves differently modulated other metabolic switches, leading to significant changes in the levels of sugars, amino acids, and organic acids in transgenic plants under control and stress conditions. Our findings reveal a novel potential technological solution to tackle multiple stresses under changing climatic conditions.