ARABIDOPSIS CDNA SEQUENCE ENCODING MYROSINASE
ARABIDOPSIS CDNA SEQUENCE ENCODING MYROSINASE
复制标题
DOI：
10.1104/pp.103.2.671
复制
发表时间：
1993-10-01
期刊：
影响因子：
7.4
通讯作者：
BRADLEY, D
中科院分区：
文献类型：
--
作者：
CHADCHAWAN, S;BISHOP, J;BRADLEY, D
黑芥子酶（0-硫代葡萄糖苷酶，硫代葡萄糖苷葡萄糖水解酶，EC 3.2。3.1)催化芥子油苷的水解，芥子油苷是十字花科植物中存在的一类含硫糖苷。虽然完整的芥子油苷相对无毒，但它们的分解产物（异硫氰酸酯、腈或硫氰酸酯）对哺乳动物、昆虫和微生物病原体具有重要的生物学影响（综述参见芬威克et al.，1983年）。黑芥子酶已从许多十字花科物种中纯化，发现是一种135，000 mo 1 wt的糖蛋白，由两个65，000 mo 1 wt的亚基组成（Bjorkman，1976; Bones和Slupphaug，1989）。在完整的植物组织中，黑芥子酶被隔离在称为黑芥子细胞的专门细胞中。当植物组织由于病原体或草食动物的攻击而受损时，黑芥子酶和硫代葡萄糖苷接触，导致硫代葡萄糖苷水解为生物活性化合物。白芥和欧洲油菜的黑芥子酶cDNA序列已被报道（Falk等人，1992; Xue等人，1992年）。Southern杂交分析了S. alba（Xue等人，1992），B.欧洲油菜（Falk等，1992）和芜菁（Brassica rapa（S. Machlin和D.布拉德利，未发表的结果）已经表明，在这些物种中，黑芥子酶由6至14个基因组成的大的多基因家族编码。然而，“这项工作得到了华盛顿技术中心（DB和TM-O.）的资助，华盛顿大学研究生研究基金（DB和JB）和国家科学基金会，BSR-9022025（TM-O. DB）。SC得到了Ananthamahidol基金会的支持。通讯作者;传真：1-206-543-3262。
Myrosinase (0-thioglucosidase, thioglucoside glucohydrolase, EC 3.2. 3.1) catalyzes the hydrolysis of glucosinolates, a class of sulfur-containing glycosides present in a11 Crucifers. Although intact glucosinolates are relatively nontoxic, their breakdown products (isothiocyanates, nitriles, or thiocyanates) have important biological influences on mammals, insects, and microbial pathogens (for review, see Fenwick et al., 1983). Myrosinase has been purified from a number of Crucifer species and found to be a glycoprotein of 135,000 mo1 wt consisting of two subunits of 65,000 mo1 wt (Bjorkman, 1976; Bones and Slupphaug, 1989). In intact plant tissues, myrosinase is sequestered in specialized cells called myrosin cells. When plant tissues are damaged as the result of pathogen or herbivore attack, myrosinase and glucosinolates come into contact, causing hydrolysis of glucosinolates to the biologically active compounds. Myrosinase cDNA sequences have been reported for Sinapis alba and Brassica napus (Falk et al., 1992; Xue et al., 1992). Southern blot analysis of genomic DNA isolated from S. alba (Xue et al., 1992), B. napus (Falk et al., 1992), and Brassica rapa (S. Machlin and D. Bradley, unpublished results) has shown that in these species myrosinase is encoded by large multigene families consisting of 6 to 14 genes. However,'This work was supported by funding from the Washington Technology Center (DB and TM-O.), the University of Washington Graduate Research Fund (DB and JB), and the National Science Foundation, BSR-9022025 (TM-O. and DB). SC was supported by the Ananthamahidol Foundation.* Corresponding author; fax 1-206-543-3262.