Cell separation between mesenchymal progenitor cells through porous polymeric membranes.
Cell separation between mesenchymal progenitor cells through porous polymeric membranes.
复制标题
通过多孔聚合物膜实现间充质祖细胞之间的细胞分离。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
A. Umezawa
中科院分区：
文献类型：
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作者：
A. Higuchi;Y. Shindo;Yumiko Gomei;Taisuke Mori;T. Uyama;A. Umezawa
本研究探讨了两种类型的骨髓基质细胞，KUSA-A1成骨细胞和H-1/A前脂肪细胞的分离，通过过滤通过各种多孔聚合物膜。发现KUSA-A1比H-1/A细胞更好地透过12微米聚氨酯泡沫膜。这似乎是由于KUSA-A1细胞的细胞尺寸相对较小。此外，当使用含有细胞类型或两者的混合物的悬浮液的进料溶液时，通过聚氨酯和表面改性的聚氨酯发泡膜的渗透率相对较低（< 6%）。还发现，用尼龙网过滤膜在KUSA-A1和H-1/A细胞之间有一定程度的分离（分离因子= 1.8），但当使用由非织造织物或丝网制成的过滤器时没有获得分离。尼龙网过滤膜分离两种细胞类型的能力是由于最佳孔径导致的筛分效应。最后，在过滤细胞后，人血清白蛋白溶液通过膜的渗透不会导致回收溶液中细胞的分离。
This study investigates the separation of two types of marrow stromal cells, KUSA-A1 osteoblasts and H-1/A preadipocytes, by filtration through various porous polymeric membranes. It was found that KUSA-A1 permeates better than H-1/A cells through 12-microm polyurethane foaming membranes. This appears to be due to the relatively smaller cell size of KUSA-A1 cells. In addition, when feed solutions containing suspensions of either cell type or a mixture of the two were used, the permeation ratio was relatively low (< 6%) through polyurethane and surface-modified polyurethane foaming membranes. It was also found that there was some degree of separation between KUSA-A1 and H-1/A cells (separation factor = 1.8) with nylon-net filter membranes, but no separation was obtained when filters made of nonwoven fabrics or silk screens were used. This ability of the nylon-net filter membranes to separate the two cell types was due to a sieving effect that results from an optimal pore size. Finally, permeation of a solution of human serum albumin through the membrane following filtration of the cells did not result in a separation of cells in the recovery solution.
DOI：
10.1002/1097-0320(20011201)45:4
发表时间：
2001-12-01
期刊：
CYTOMETRY
影响因子：
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作者：
Comella, K;Nakamura, M;Chalmers, JJ
通讯作者：
Chalmers, JJ